Evidence map›Paper›PMID 25941157›Full record

ArticlePlant cell reports2015

Isolation of a maize ZmCI-1B promoter and characterization of its activity in transgenic maize and tobacco.

Ye Li, Xiaoqing Liu, Jie Li, Suzhen Li, Guanyu Chen, Xiaojin Zhou, Wenzhu Yang, Rumei Chen

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Article in Plant cell reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.3field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 6 institutions in 1 country.

Ye LiDepartment of Crop Genomics and Genetic Improvement, Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, 12 ZhongGuanCun South Street, Beijing, 100081, China.
Xiaoqing Liu
Jie Li
Suzhen Li
Guanyu Chen
Xiaojin Zhou
Wenzhu Yang
Rumei Chen
Chinese Academy of Agricultural Sciences · CNBeijing Normal University · CNBiotechnology Research Institute · CNHebei Agricultural University · CNInstitute of Crop Sciences · CNRenmin University of China · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

key messageThe 2-kb ZmCI - 1B promoter is active in the root and embryo and induced by wounding in maize and the 220-bp 5'-deleted segment maybe the minimal promoter. The subtilisin-chymotrypsin inhibitor gene, CI-1B of Zea mays (ZmCI-1B), has been suggested to induce the maize defense system to resist insect attack. Real-time RT-PCR showed that ZmCI-1B gene exhibited especially high expression in roots and embryos. The 2-kb full-length promoter of ZmCI-1B gene was isolated from the maize genome and used to drive expression of a beta-glucuronidase (GUS) reporter gene for transient expression and stable expression analysis in maize. The results of GUS histochemical staining in transgenic maize plants revealed that the ZmCI-1B promoter induced GUS expression preferentially in roots and embryos and in response to wounding. A series of 5'-deleted segments of the ZmCI-1B promoter were cloned individually to drive GUS expression for further analysis. Deletion analysis combined with the histochemical staining of transgenic tobacco plants revealed 220-bp segment could drive GUS in a tissue-specific and wounding-induced expression in tobacco; thus, it maybe the minimally active promoter of ZmCI-1B gene. Furthermore, it revealed that the ZmCI-1B promoter contained tissue-specific and wounding-induced elements.

Indexed as

ChymotrypsinGene Expression Regulation, PlantGlucuronidaseNicotianaPlant ProteinsPlant RootsPlants, Genetically ModifiedPromoter Regions, GeneticReal-Time Polymerase Chain ReactionSubtilisinZea maysChymotrypsinGlucuronidasePlant ProteinsSubtilisin

Identifiers

PMID25941157
OpenAlexW315709188

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.