ArticleGenes to cells : devoted to molecular & cellular mechanisms2015
Phenotype-based clustering of glycosylation-related genes by RNAi-mediated gene silencing.
Article in Genes to cells : devoted to molecular & cellular mechanisms, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- N-Glycoengineering of insect cells for tri-antennary N-glycan biosynthesis.Scientific reports · 2026Article
- Glycan-related genes and genetic disorders.Journal of human genetics · 2026Review
- RNAi-based screen for pigmentation iniScience · 2026Article
- RNAi-based screen for pigmentation inbioRxiv : the preprint server for biology · 2025Article
- Neuromuscular Defects in aBiomolecules · 2025Article
- Glycosylation in Stem Cell Biology.Handbook of experimental pharmacology · 2025Review
- Review
- Generation of Mutants from the 57B Region ofGenes · 2023Article
- Regulation of morphogen pathways by a Drosophila chondroitin sulfate proteoglycan Windpipe.Journal of cell science · 2023Article
- Hemocyte Clusters Defined by scRNA-Seq inFrontiers in immunology · 2022Article
- Mucin-TypeFrontiers in neuroanatomy · 2021Article
- RNAi-Mediated Silencing of Pgants Shows Core 1Frontiers in physiology · 2021Article
- Functional analysis of glycosylation using Drosophila melanogaster.Glycoconjugate journal · 2020Review
- Synthesis and biological roles of O-glycans in insects.Glycoconjugate journal · 2020Review
- Protein N-glycosylation and N-glycan trimming are required for postembryonic development of the pest beetle Tribolium castaneum.Scientific reports · 2016Article
- Targeted release and fractionation reveal glucuronylated and sulphated N- and O-glycans in larvae of dipteran insects.Journal of proteomics · 2015Article
- Sugar tags and tumorigenesis.Frontiers in cell and developmental biology · 2015Article
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13 authors.
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Abstract
Glycan structures are synthesized by a series of reactions conducted by glycosylation-related (GR) proteins such as glycosyltransferases, glycan-modifying enzymes, and nucleotide-sugar transporters. For example, the common core region of glycosaminoglycans (GAGs) is sequentially synthesized by peptide-O-xylosyltransferase, β1,4-galactosyltransferase I, β1,3-galactosyltransferase II, and β1,3-glucuronyltransferase. This raises the possibility that functional impairment of GR proteins involved in synthesis of the same glycan might result in the same phenotypic abnormality. To examine this possibility, comprehensive silencing of genes encoding GR and proteoglycan core proteins was conducted in Drosophila. Drosophila GR candidate genes (125) were classified into five functional groups for synthesis of GAGs, N-linked, O-linked, Notch-related, and unknown glycans. Spatiotemporally regulated silencing caused a range of malformed phenotypes that fell into three types: extra veins, thick veins, and depigmentation. The clustered phenotypes reflected the biosynthetic pathways of GAGs, Fringe-dependent glycan on Notch, and glycans placed at or near nonreducing ends (herein termed terminal domains of glycans). Based on the phenotypic clustering, CG33145 was predicted to be involved in formation of terminal domains. Our further analysis showed that CG33145 exhibited galactosyltransferase activity in synthesis of terminal N-linked glycans. Phenotypic clustering, therefore, has potential for the functional prediction of novel GR genes.
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