ArticleScientific reports2015
Enhancing the light-driven production of D-lactate by engineering cyanobacterium using a combinational strategy.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 59 citations in OpenAlex.
- Cyanobacterial conversion of COChemical science · 2026Review
- Photovoltaic Microorganism Hybrid Systems for Enhanced Polyhydroxybutyrate Synthesis Through Material Design and Energy Mass Transfer Mechanisms.Materials (Basel, Switzerland) · 2025Review
- Methanol biotransformation for the production of biodegradable plastic monomer L-lactate in yeast.Nature communications · 2025Article
- Circular cell culture for sustainable food production using recombinant lactate-assimilating cyanobacteria that supplies pyruvate and amino acids.Archives of microbiology · 2023Article
- L-Lactate treatment by photosynthetic cyanobacteria expressing heterogeneous L-lactate dehydrogenase.Scientific reports · 2023Article
- Light-Driven Synthetic Biology: Progress in Research and Industrialization of Cyanobacterial Cell Factory.Life (Basel, Switzerland) · 2022Review
- Autotrophic lactate production from HApplied microbiology and biotechnology · 2022Article
- Exploitation of Hetero- and Phototrophic Metabolic Modules for Redox-Intensive Whole-Cell Biocatalysis.Frontiers in bioengineering and biotechnology · 2022Review
- Over-expression of an electron transport protein OmcS provides sufficient NADH for D-lactate production in cyanobacterium.Biotechnology for biofuels · 2021Article
- Genetic, Genomics, and Responses to Stresses in Cyanobacteria: Biotechnological Implications.Genes · 2021Review
- Enhanced Production of D-Lactate in Cyanobacteria by Re-Routing Photosynthetic Cyclic and Pseudo-Cyclic Electron Flow.Frontiers in plant science · 2019Article
- Engineering Microbial Consortia for High-Performance Cellulosic Hydrolyzates-Fed Microbial Fuel Cells.Frontiers in microbiology · 2019Article
- Development of a High-Throughput, In Vivo Selection Platform for NADPH-Dependent Reactions Based on Redox Balance Principles.ACS synthetic biology · 2018Article
- Substrate Specificity and Allosteric Regulation of a D-Lactate Dehydrogenase from a Unicellular Cyanobacterium are Altered by an Amino Acid Substitution.Scientific reports · 2017Article
- EngineeringBiotechnology for biofuels · 2017Article
- Engineering a d-lactate dehydrogenase that can super-efficiently utilize NADPH and NADH as cofactors.Scientific reports · 2016Article
- Chirality Matters: Synthesis and Consumption of the d-Enantiomer of Lactic Acid by Synechocystis sp. Strain PCC6803.Applied and environmental microbiology · 2016Article
- Advances in Metabolic Engineering of Cyanobacteria for Photosynthetic Biochemical Production.Metabolites · 2015Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It is increasingly attractive to engineer cyanobacteria for bulk production of chemicals from CO2. However, cofactor bias of cyanobacteria is different from bacteria that prefer NADH, which hampers cyanobacterial strain engineering. In this study, the key enzyme d-lactate dehydrogenase (LdhD) from Lactobacillus bulgaricus ATCC11842 was engineered to reverse its favored cofactor from NADH to NADPH. Then, the engineered enzyme was introduced into Synechococcus elongatus PCC7942 to construct an efficient light-driven system that produces d-lactic acid from CO2. Mutation of LdhD drove a fundamental shift in cofactor preference towards NADPH, and increased d-lactate productivity by over 3.6-fold. We further demonstrated that introduction of a lactic acid transporter and bubbling CO2-enriched air also enhanced d-lactate productivity. Using this combinational strategy, increased d-lactate concentration and productivity were achieved. The present strategy may also be used to engineer cyanobacteria for producing other useful chemicals.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.