ArticleInfection and immunity2015
Identification of SlpB, a Cytotoxic Protease from Serratia marcescens.
Article in Infection and immunity, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 46 citations in OpenAlex.
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- Regulation of indigoidine production inJournal of bacteriology · 2026Article
- Generation and comparison of broad-host range inducible expression vectors for use in Gram-negative bacteria including ESKAPE pathogens.Proceedings of the West Virginia Academy of Science · 2025Article
- Recombinant serralysin metalloproteases D enhances the intracellular replication of infectious bovine rhinotracheitis virus.Frontiers in microbiology · 2025Article
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- Increased Proteolytic Activity ofPolish journal of microbiology · 2024Article
- Clonal dissemination of highly virulentOne health (Amsterdam, Netherlands) · 2023Article
- Ocular Bacterial Infections: A Ten-Year Survey and Review of Causative Organisms Based on the Oklahoma Experience.Microorganisms · 2023Article
- Identification of a cytotoxic factor from a non-pigmented entomopathogenic Serratia marcescens isolate toxic towards human carcinoma cell lines.Archives of microbiology · 2023Article
- Evaluation ofMicroorganisms · 2023Article
- Proteogenomic analysis of Serratia marcescens using computational subtractive genomics approach.PloS one · 2023Article
- IgaA Protein, GumB, Has a Global Impact on the Transcriptome and Surface Proteome of Serratia marcescens.Infection and immunity · 2022Article
- A Functional Analysis of the Purine Salvage Pathway in Acetobacter fabarum.Journal of bacteriology · 2022Article
- The fliR gene contributes to the virulence of S. marcescens in a Drosophila intestinal infection model.Scientific reports · 2022Article
- The Role of SprIR Quorum Sensing System in the Regulation ofMicroorganisms · 2021Article
- Clearance of Gram-Negative Bacterial Pathogens from the Ocular Surface by Predatory Bacteria.Antibiotics (Basel, Switzerland) · 2021Article
- Transcription Factor EepR Is Required forAntibiotics (Basel, Switzerland) · 2021Article
- [Serratia marcescens as cause of delayed onset endophtalmitis].Revista espanola de quimioterapia : publicacion oficial de la Sociedad Espanola de Quimioterapia · 2021Article
- Differential susceptibility of airway and ocular surface cell lines to FlhDC-mediated virulence factors PhlA and ShlA fromJournal of medical microbiology · 2021Article
- Biologically active pigment and ShlA cytolysin of Serratia marcescens induce autophagy in a human ocular surface cell line.BMC ophthalmology · 2020Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The Gram-negative bacterium and opportunistic pathogen Serratia marcescens causes ocular infections in healthy individuals. Secreted protease activity was characterized from 44 ocular clinical isolates, and a higher frequency of protease-positive strains was observed among keratitis isolates than among conjunctivitis isolates. A positive correlation between protease activity and cytotoxicity to human corneal epithelial cells in vitro was determined. Deletion of prtS in clinical keratitis isolate K904 reduced, but did not eliminate, cytotoxicity and secreted protease production. This indicated that PrtS is necessary for full cytotoxicity to ocular cells and implied the existence of another secreted protease(s) and cytotoxic factors. Bioinformatic analysis of the S. marcescens Db11 genome revealed three additional open reading frames predicted to code for serralysin-like proteases noted here as slpB, slpC, and slpD. Induced expression of prtS and slpB, but not slpC and slpD, in strain PIC3611 rendered the strain cytotoxic to a lung carcinoma cell line; however, only prtS induction was sufficient for cytotoxicity to a corneal cell line. Strain K904 with deletion of both prtS and slpB genes was defective in secreted protease activity and cytotoxicity to human cell lines. PAGE analysis suggests that SlpB is produced at lower levels than PrtS. Purified SlpB demonstrated calcium-dependent and AprI-inhibited protease activity and cytotoxicity to airway and ocular cell lines in vitro. Lastly, genetic analysis indicated that the type I secretion system gene, lipD, is required for SlpB secretion. These genetic data introduce SlpB as a new cytotoxic protease from S. marcescens.
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