Evidence map›Paper›PMID 25939389›Full record

ArticleJournal of cellular biochemistry2015

A distinct role for interleukin-6 as a major mediator of cellular adjustment to an altered culture condition.

Hwa-Kyung Son, Iha Park, Jue Young Kim, Do Kyeong Kim, Rasika P Illeperuma, Jung Yoon Bae, Doo Young Lee, Eun-Sang Oh, Da-Woon Jung, Darren R Williams and 1 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of cellular biochemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.1field-weighted citation impact, top 44% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 3 citations in OpenAlex.

  1. Review
  2. The Protective Effect of Antioxidants in Areca Nut Extract-Induced Oral Carcinogenesis.Asian Pacific journal of cancer prevention : APJCP · 2020
    Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 4 institutions in 2 countries.

Hwa-Kyung SonDepartment of Dental Hygiene, Division of Health science, Yeungnam University College, Daegu, Korea.
Iha ParkChonnam National University Research Institute of Medical Sciences, Gwangju, Korea.
Jue Young KimDepartment of Oral Pathology, Oral Cancer Research Institute, Brain Korea 21 Plus Project, Yonsei University, College of Dentistry, Seoul, Korea.
Do Kyeong KimDepartment of Oral Pathology, Oral Cancer Research Institute, Brain Korea 21 Plus Project, Yonsei University, College of Dentistry, Seoul, Korea.
Rasika P IlleperumaDepartment of Medical Laboratory Science, Faculty of Allied Health Sciences, University of Peradeniya, Sri Lanka.
Jung Yoon BaeDepartment of Oral Pathology, Oral Cancer Research Institute, Brain Korea 21 Plus Project, Yonsei University, College of Dentistry, Seoul, Korea.
Doo Young LeeDepartment of Oral Pathology, Oral Cancer Research Institute, Brain Korea 21 Plus Project, Yonsei University, College of Dentistry, Seoul, Korea.
Eun-Sang OhNew Drug Targets Laboratory, School of Life Sciences, Gwangju Institute of Science and Technology, Gwangju, 500-712, Korea.
Da-Woon JungNew Drug Targets Laboratory, School of Life Sciences, Gwangju Institute of Science and Technology, Gwangju, 500-712, Korea.
Darren R WilliamsNew Drug Targets Laboratory, School of Life Sciences, Gwangju Institute of Science and Technology, Gwangju, 500-712, Korea.
Jin KimDepartment of Oral Pathology, Oral Cancer Research Institute, Brain Korea 21 Plus Project, Yonsei University, College of Dentistry, Seoul, Korea.
Yonsei University · KRGwangju Institute of Science and Technology · KRUniversity of Peradeniya · LKChonnam National University · KR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Tissue microenvironment adjusts biological properties of different cells by modulating signaling pathways and cell to cell interactions. This study showed that epithelial-mesenchymal transition (EMT)/ mesenchymal-epithelial transition (MET) can be modulated by altering culture conditions. HPV E6/E7-transfected immortalized oral keratinocytes (IHOK) cultured in different media displayed reversible EMT/MET accompanied by changes in cell phenotype, proliferation, gene expression at transcriptional, and translational level, and migratory and invasive activities. Cholera toxin, a major supplement to culture medium, was responsible for inducing the morphological and biological changes of IHOK. Cholera toxin per se induced EMT by triggering the secretion of interleukin 6 (IL-6) from IHOK. We found IL-6 to be a central molecule that modulates the reversibility of EMT based not only on the mRNA level but also on the level of secretion. Taken together, our results demonstrate that IL-6, a cytokine whose transcription is activated by alterations in culture conditions, is a key molecule for regulating reversible EMT/MET. This study will contribute to understand one way of cellular adjustment for surviving in unfamiliar conditions.

Indexed as

AnimalsCell Culture TechniquesCell LineCell MovementCell ProliferationCellular MicroenvironmentCholera ToxinCulture MediaEpithelial-Mesenchymal TransitionHumansInterleukin-6KeratinocytesMCF-7 CellsNeoplasm TransplantationPhenotypeUp-RegulationCholera ToxinCulture MediaIL6 protein, humanInterleukin-6CHOLERA TOXINCULTURE CONDITIONEPITHELIAL MESENCHYMAL TRANSITION (EMT)IL-6MESENCHYMAL EPITHELIAL TRANSITION (MET)

Identifiers

PMID25939389
PMCPMC4832257
OpenAlexW2172190434

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.