ArticleThe Plant journal : for cell and molecular biology2015
Elucidating steroid alkaloid biosynthesis in Veratrum californicum: production of verazine in Sf9 cells.
Article in The Plant journal : for cell and molecular biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 82 citations in OpenAlex.
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- Advances in the microbial biosynthesis of therapeutic terpenoids.Current opinion in biotechnology · 2025Review
- Oncogenic B-Raf proto-oncogene, serine/threonine kinase-mediated hedgehog signalling in the pathogenesis and targeted therapy of melanoma.World journal of clinical oncology · 2025Review
- The clarification of two hydroxylation steps in the conversion of cholesterol to OSW-1 in Ornithogalum saundersiae.Plant biotechnology journal · 2025Article
- Identification of genes involved in verazine biosynthesis in Veratrum grandiflorum and their heterologous production in Saccharomyces cerevisiae.BMC plant biology · 2025Article
- Two reductases complete steroidal glycoalkaloids biosynthesis in potato.The New phytologist · 2025Article
- Recent advancements in the synthesis ofBeilstein journal of organic chemistry · 2025Review
- Incorporation of nitrogen in antinutritional Solanum alkaloid biosynthesis.Nature chemical biology · 2025Article
- A developmental gradient reveals biosynthetic pathways to eukaryotic toxins in monocot geophytes.Cell · 2024Article
- Verazine biosynthesis from simple sugars in engineered Saccharomyces cerevisiae.Metabolic engineering · 2024Article
- Integrative analysis of metabolome and transcriptome provides new insights into functional components ofChinese herbal medicines · 2024Article
- Efficient heterologous biosynthesis of verazine, a metabolic precursor of the anti-cancer drug cyclopamine, in Nicotiana benthamiana.Plant communications · 2024Article
- Transcriptomic analysis reveals effects of fertilization towards growth and quality of Fritillariae thunbergii bulbus.PloS one · 2024Article
- Comparative transcriptome analysis of Veratrum maackii and Veratrum nigrum reveals multiple candidate genes involved in steroidal alkaloid biosynthesis.Scientific reports · 2023Article
- Steroidal saponin profiles and their key genes for synthesis and regulation in Asparagus officinalis L. by joint analysis of metabolomics and transcriptomics.BMC plant biology · 2023Article
- Article
- Transcriptome and metabolome profiling of the medicinal plantFrontiers in genetics · 2023Article
- Review
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Authors and funding
17 authors at 10 institutions in 5 countries.
Funding
Abstract
Steroid alkaloids have been shown to elicit a wide range of pharmacological effects that include anticancer and antifungal activities. Understanding the biosynthesis of these molecules is essential to bioengineering for sustainable production. Herein, we investigate the biosynthetic pathway to cyclopamine, a steroid alkaloid that shows promising antineoplastic activities. Supply of cyclopamine is limited, as the current source is solely derived from wild collection of the plant Veratrum californicum. To elucidate the early stages of the pathway to cyclopamine, we interrogated a V. californicum RNA-seq dataset using the cyclopamine accumulation profile as a predefined model for gene expression with the pattern-matching algorithm Haystack. Refactoring candidate genes in Sf9 insect cells led to discovery of four enzymes that catalyze the first six steps in steroid alkaloid biosynthesis to produce verazine, a predicted precursor to cyclopamine. Three of the enzymes are cytochromes P450 while the fourth is a γ-aminobutyrate transaminase; together they produce verazine from cholesterol.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.