ArticleJournal of visualized experiments : JoVE2015
Robotic production of cancer cell spheroids with an aqueous two-phase system for drug testing.
Article in Journal of visualized experiments : JoVE, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed.
- Inhibiting BRAF/EGFR/MEK suppresses cancer stemness and drug resistance of primary colorectal cancer cells.Oncotarget · 2023Article
- Therapeutic Targeting of Cancer Stem Cells Prevents Resistance of Colorectal Cancer Cells to MEK Inhibition.ACS pharmacology & translational science · 2022Article
- Engineering of hybrid spheroids of mesenchymal stem cells and drug depots for immunomodulating effect in islet xenotransplantation.Science advances · 2022Article
- Two-Dimensional and Three-Dimensional Cartilage Model Platforms for Drug Evaluation and High-Throughput Screening Assays.Tissue engineering. Part B, Reviews · 2022Review
- Modeling adaptive drug resistance of colorectal cancer and therapeutic interventions with tumor spheroids.Experimental biology and medicine (Maywood, N.J.) · 2021Review
- 3D cell aggregate printing technology and its applications.Essays in biochemistry · 2021Review
- Mapping cellular-scale internal mechanics in 3D tissues with thermally responsive hydrogel probes.Nature communications · 2020Article
- Three-dimensional models of breast cancer-fibroblasts interactions.Experimental biology and medicine (Maywood, N.J.) · 2020Review
- Organotypic breast tumor model elucidates dynamic remodeling of tumor microenvironment.Biomaterials · 2020Article
- Gold Nanoparticles Mediated Drug-Gene Combinational Therapy for Breast Cancer Treatment.International journal of nanomedicine · 2020Article
- Synergistic Inhibition of Kinase Pathways Overcomes Resistance of Colorectal Cancer Spheroids to Cyclic Targeted Therapies.ACS pharmacology & translational science · 2019Article
- Quantitative Size-Based Analysis of Tumor Spheroids and Responses to Therapeutics.Assay and drug development technologies · 2019Article
- Dispersible hydrogel force sensors reveal patterns of solid mechanical stress in multicellular spheroid cultures.Nature communications · 2019Article
- Biomaterials-Based Approaches to Tumor Spheroid and Organoid Modeling.Advanced healthcare materials · 2018Review
- Article
- Opportunities and challenges in phenotypic drug discovery: an industry perspective.Nature reviews. Drug discovery · 2017Review
- The third dimension: new developments in cell culture models for colorectal research.Cellular and molecular life sciences : CMLS · 2016Review
- Engineered Breast Cancer Cell Spheroids Reproduce Biologic Properties of Solid Tumors.Advanced healthcare materials · 2016Article
- Liquid-based three-dimensional tumor models for cancer research and drug discovery.Experimental biology and medicine (Maywood, N.J.) · 2016Review
- Phytochemicals potently inhibit migration of metastatic breast cancer cells.Integrative biology : quantitative biosciences from nano to macro · 2015Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Cancer cell spheroids present a relevant in vitro model of avascular tumors for anti-cancer drug testing applications. A detailed protocol for producing both mono-culture and co-culture spheroids in a high throughput 96-well plate format is described in this work. This approach utilizes an aqueous two-phase system to confine cells into a drop of the denser aqueous phase immersed within the second aqueous phase. The drop rests on the well surface and keeps cells in close proximity to form a single spheroid. This technology has been adapted to a robotic liquid handler to produce size-controlled spheroids and expedite the process of spheroid production for compound screening applications. Spheroids treated with a clinically-used drug show reduced cell viability with increase in the drug dose. The use of a standard micro-well plate for spheroid generation makes it straightforward to analyze viability of cancer cells of drug-treated spheroids with a micro-plate reader. This technology is straightforward to implement both robotically and with other liquid handling tools such as manual pipettes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.