ArticleJournal of visualized experiments : JoVE2015
An inexpensive, scalable behavioral assay for measuring ethanol sedation sensitivity and rapid tolerance in Drosophila.
Article in Journal of visualized experiments : JoVE, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 14 citations in OpenAlex.
- Dual In Vivo and In Silico Exploration of Jet A‑1 Fuel Toxicity.ACS omega · 2026Article
- Diet Modification Alters Repeated Exposure Ethanol Tolerance Development.microPublication biology · 2026Article
- Long term exposure to dietary cannabinoids inhibits rapid functional tolerance to ethanol inmicroPublication biology · 2025Article
- TBI and Tau Loss of Function Both Affect Naïve Ethanol Sensitivity inInternational journal of molecular sciences · 2024Article
- Microbiome-by-ethanol interactions impact Drosophila melanogaster fitness, physiology, and behavior.iScience · 2022Article
- Identification of additional dye tracers for measuring solid food intake and food preference via consumption-excretion in Drosophila.Scientific reports · 2022Article
- Corazonin Neurons Contribute to Dimorphic Ethanol Sedation Sensitivity inFrontiers in neural circuits · 2022Article
- The Serine Protease Homolog, Scarface, Is Sensitive to Nutrient Availability and Modulates the Development of theThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- Repeated ethanol intoxications of Drosophila melanogaster adults increases the resistance to ethanol of their progeny.Alcoholism, clinical and experimental research · 2021Article
- Tyramine synthesis, vesicular packaging, and the SNARE complex function coordinately in astrocytes to regulate Drosophila alcohol sedation.Addiction biology · 2021Article
- Dietary yeast influences ethanol sedation in Drosophila via serotonergic neuron function.Addiction biology · 2020Article
- High-Throughput Method for Measuring Alcohol Sedation Time of Individual Drosophila melanogaster.Journal of visualized experiments : JoVE · 2020Article
- Convergent Evidence From Humans and Drosophila melanogaster Implicates the Transcription Factor MEF2B/Mef2 in Alcohol Sensitivity.Alcoholism, clinical and experimental research · 2019Article
- Alcohol sedation in adultCommunications biology · 2019Article
- Drosophila and Caenorhabditis elegans as Discovery Platforms for Genes Involved in Human Alcohol Use Disorder.Alcoholism, clinical and experimental research · 2015Review
- G-protein αq gene expression plays a role in alcohol tolerance inBrain and neuroscience advancesArticle
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Alcohol use disorder (AUD) is a serious health challenge. Despite a large hereditary component to AUD, few genes have been unambiguously implicated in their etiology. The fruit fly, Drosophila melanogaster, is a powerful model for exploring molecular-genetic mechanisms underlying alcohol-related behaviors and therefore holds great promise for identifying and understanding the function of genes that influence AUD. The use of the Drosophila model for these types of studies depends on the availability of assays that reliably measure behavioral responses to ethanol. This report describes an assay suitable for assessing ethanol sensitivity and rapid tolerance in flies. Ethanol sensitivity measured in this assay is influenced by the volume and concentration of ethanol used, a variety of previously reported genetic manipulations, and also the length of time the flies are housed without food immediately prior to testing. In contrast, ethanol sensitivity measured in this assay is not affected by the vigor of fly handling, sex of the flies, and supplementation of growth medium with antibiotics or live yeast. Three different methods for quantitating ethanol sensitivity are described, all leading to essentially indistinguishable ethanol sensitivity results. The scalable nature of this assay, combined with its overall simplicity to set-up and relatively low expense, make it suitable for small and large scale genetic analysis of ethanol sensitivity and rapid tolerance in Drosophila.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.