ArticleJournal of visualized experiments : JoVE2015
Synchronization of Caulobacter crescentus for investigation of the bacterial cell cycle.
Article in Journal of visualized experiments : JoVE, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
31 citing papers in PubMed.
- The complete genome sequence ofMicrobiology resource announcements · 2026Article
- Stress changes the material state of a bacterial biomolecular condensate and shifts its function from mRNA decay to storage.Nature communications · 2025Article
- Stimulation of themBio · 2025Article
- BR-bodies facilitate adaptive responses and survival during copper stress in Caulobacter crescentus.The Journal of biological chemistry · 2025Article
- Upstream CtrA-binding sites both induce and repress pilin gene expression in Caulobacter crescentus.BMC genomics · 2024Article
- GTPase activity regulates FtsZ ring positioning inMolecular biology of the cell · 2024Article
- Cell cycle dependent coordination of surface layer biogenesis in Caulobacter crescentus.Nature communications · 2024Article
- Role ofMicroorganisms · 2024Article
- Influence of the Heme Nitric Oxide/Oxygen Binding Protein (H-NOX) on Cell Cycle Regulation in Caulobacter crescentus.Molecular & cellular proteomics : MCP · 2023Article
- The BR-body proteome contains a complex network of protein-protein and protein-RNA interactions.Cell reports · 2023Article
- The BR-body proteome contains a complex network of protein-protein and protein-RNA interactions.bioRxiv : the preprint server for biology · 2023Article
- Scaffold-Scaffold Interaction Facilitates Cell Polarity Development in Caulobacter crescentus.mBio · 2023Article
- Turing-pattern model of scaffolding proteins that establish spatial asymmetry during the cell cycle ofiScience · 2023Article
- Synchronized Swarmers and Sticky Stalks: Caulobacter crescentus as a Model for Bacterial Cell Biology.Journal of bacteriology · 2023Review
- Article
- Programmed Flagellar Ejection in Caulobacter crescentus Leaves PL-subcomplexes.Journal of molecular biology · 2021Article
- Article
- A localized adaptor protein performs distinct functions at theProceedings of the National Academy of Sciences of the United States of America · 2021Article
- BR-Bodies Provide Selectively Permeable Condensates that Stimulate mRNA Decay and Prevent Release of Decay Intermediates.Molecular cell · 2020Article
- Untargeted metabolomics links glutathione to bacterial cell cycle progression.Nature metabolism · 2020Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
The cell cycle is important for growth, genome replication, and development in all cells. In bacteria, studies of the cell cycle have focused largely on unsynchronized cells making it difficult to order the temporal events required for cell cycle progression, genome replication, and division. Caulobacter crescentus provides an excellent model system for the bacterial cell cycle whereby cells can be rapidly synchronized in a G0 state by density centrifugation. Cell cycle synchronization experiments have been used to establish the molecular events governing chromosome replication and segregation, to map a genetic regulatory network controlling cell cycle progression, and to identify the establishment of polar signaling complexes required for asymmetric cell division. Here we provide a detailed protocol for the rapid synchronization of Caulobacter NA1000 cells. Synchronization can be performed in a large-scale format for gene expression profiling and western blot assays, as well as a small-scale format for microscopy or FACS assays. The rapid synchronizability and high cell yields of Caulobacter make this organism a powerful model system for studies of the bacterial cell cycle.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.