ArticleJournal of visualized experiments : JoVE2015
An optimized enrichment technique for the isolation of Arthrobacter bacteriophage species from soil sample isolates.
Article in Journal of visualized experiments : JoVE, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 30 citations in OpenAlex.
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- Statistical optimization of a podoviral anti-MRSA phage CCASU-L10 generated from an under sampled repository: Chicken rinse.Frontiers in cellular and infection microbiology · 2023Article
- Isolation and Characterization of Lytic Bacteriophages Active against Clinical Strains ofViruses · 2022Article
- Complete Genome Sequence of Dickeya chrysanthemi Bacteriophage DchS19.Microbiology resource announcements · 2022Article
- Viruses direct carbon cycling in lake sediments under global change.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Isolation and Characterization of LyticViruses · 2022Article
- Heterogeneity of soil bacterial and bacteriophage communities in three rice agroecosystems and potential impacts of bacteriophage on nutrient cycling.Environmental microbiome · 2022Article
- An Appraisal of Bacteriophage Isolation Techniques from Environment.Microbial ecology · 2022Review
- Phages for Africa: The Potential Benefit and Challenges of Phage Therapy for the Livestock Sector in Sub-Saharan Africa.Antibiotics (Basel, Switzerland) · 2021Review
- Complete Genome Sequence of Stenotrophomonas maltophilia Siphophage Salva.Microbiology resource announcements · 2021Article
- Bacteriophage Treatment: Critical Evaluation of Its Application on World Health Organization Priority Pathogens.Viruses · 2020Review
- The Union Is Strength: The Synergic Action of Long Fatty Acids and a Bacteriophage againstMicroorganisms · 2020Article
- Genome Sequence ofMicrobiology resource announcements · 2020Article
- Antibiofilm Activity of aMicroorganisms · 2020Article
- Impact of phages on soil bacterial communities and nitrogen availability under different assembly scenarios.Microbiome · 2020Article
- Plant Dynamic Metabolic Response to Bacteriophage Treatment AfterFrontiers in microbiology · 2020Article
- Genome Sequences ofMicrobiology resource announcements · 2019Article
- Article
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Authors and funding
10 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacteriophage isolation from environmental samples has been performed for decades using principles set forth by pioneers in microbiology. The isolation of phages infecting Arthrobacter hosts has been limited, perhaps due to the low success rate of many previous isolation techniques, resulting in an underrepresented group of Arthrobacter phages available for study. The enrichment technique described here, unlike many others, uses a filtered extract free of contaminating bacteria as the base for indicator bacteria growth, Arthrobacter sp. KY3901, specifically. By first removing soil bacteria the target phages are not hindered by competition with native soil bacteria present in initial soil samples. This enrichment method has resulted in dozens of unique phages from several different soil types and even produced different types of phages from the same enriched soil sample isolate. The use of this procedure can be expanded to most nutrient rich aerobic media for the isolation of phages in a vast diversity of interesting host bacteria.
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