ArticlePloS one2015
Evaluation of four commonly used DNA barcoding Loci for chinese medicinal plants of the family schisandraceae.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed.
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- Comparison of the Chloroplast Genome Sequences of 13 Oil-Tea Camellia Samples and Identification of an Undetermined Oil-Tea Camellia Species From Hainan Province.Frontiers in plant science · 2021Article
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- Comparing chloroplast genomes of traditional Chinese herbsChinese herbal medicines · 2020Article
- Chloroplast (Cp) Transcriptome of P. davidiana Dode×P. bolleana Lauch provides insight into the Cp drought response and Populus Cp phylogeny.BMC evolutionary biology · 2020Article
- Re-evaluation of the phylogenetic relationships and species delimitation of two closely related families (Lamiaceae and Verbenaceae) using two DNA barcode markers.Journal of biosciences · 2020Article
- Complete chloroplast genome ofMitochondrial DNA. Part B, Resources · 2019Article
- Efficient Identification ofFrontiers in plant science · 2019Article
- DNA barcoding and TLC as tools to properly identify natural populations of the Mexican medicinal species Galphimia glauca Cav.PloS one · 2019Article
- Dynamic evolution and phylogenomic analysis of the chloroplast genome in Schisandraceae.Scientific reports · 2018Article
- A Comprehensive Comparative Study for the Authentication of theFrontiers in pharmacology · 2018Article
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- DNA barcoding evaluation and implications for phylogenetic relationships in Lauraceae from China.PloS one · 2017Article
- Efficient Identification of the Forest Tree Species in Aceraceae Using DNA Barcodes.Frontiers in plant science · 2016Article
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6 authors.
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Abstract
Many species of Schisandraceae are used in traditional Chinese medicine and are faced with contamination and substitution risks due to inaccurate identification. Here, we investigated the discriminatory power of four commonly used DNA barcoding loci (ITS, trnH-psbA, matK, and rbcL) and corresponding multi-locus combinations for 135 individuals from 33 species of Schisandraceae, using distance-, tree-, similarity-, and character-based methods, at both the family level and the genus level. Our results showed that the two spacer regions (ITS and trnH-psbA) possess higher species-resolving power than the two coding regions (matK and rbcL). The degree of species resolution increased with most of the multi-locus combinations. Furthermore, our results implied that the best DNA barcode for the species discrimination at the family level might not always be the most suitable one at the genus level. Here we propose the combination of ITS+trnH-psbA+matK+rbcL as the most ideal DNA barcode for discriminating the medicinal plants of Schisandra and Kadsura, and the combination of ITS+trnH-psbA as the most suitable barcode for Illicium species. In addition, the closely related species Schisandra rubriflora Rehder & E. H. Wilson and Schisandra grandiflora Hook.f. & Thomson, were paraphyletic with each other on phylogenetic trees, suggesting that they should not be distinct species. Furthermore, the samples of these two species from the southern Hengduan Mountains region formed a distinct cluster that was separated from the samples of other regions, implying the presence of cryptic diversity. The feasibility of DNA barcodes for identification of geographical authenticity was also verified here. The database and paradigm that we provide in this study could be used as reference for the authentication of traditional Chinese medicinal plants utilizing DNA barcoding.
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