ArticlePloS one2015
Bacterial pathogens and community composition in advanced sewage treatment systems revealed by metagenomics analysis based on high-throughput sequencing.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 91 citations in OpenAlex.
- Shotgun Metagenomic Analysis of Microbial Community Dynamics in Wastewater Treatment Through Constructed Wetlands.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Development and application of a triplex ddPCR method for simultaneous detection of MLSB resistance genes in feces and environmental samples.Frontiers in microbiology · 2026Article
- Application of a microbial and pathogen source tracking toolbox to identify infrastructure problems in stormwater drainage networks: a case study.Microbiology spectrum · 2024Article
- Occurrence, Sources and Virulence Potential ofEnvironmental science & technology · 2024Article
- Review
- Research on microbial community structure and treatment of dye wastewater with the enhancement of activated sludge by magnetic field at low temperature.RSC advances · 2023Article
- Potential Exposure to Respiratory and Enteric Bacterial Pathogens among Wastewater Treatment Plant Workers, South Africa.International journal of environmental research and public health · 2023Article
- The Prevalence ofPathogens (Basel, Switzerland) · 2022Review
- Inactivation and risk control of pathogenic microorganisms in municipal sludge treatment: A review.Frontiers of environmental science & engineering · 2022Review
- NaturalizedFrontiers in microbiology · 2022Review
- Seasonal and Technological Shifts of the WHO Priority Multi-Resistant Pathogens in Municipal Wastewater Treatment Plant and Its Receiving Surface Water: A Case Study.International journal of environmental research and public health · 2021Article
- Metagenomic profiling of antibiotic resistance and virulence removal: Activated sludge vs. algal wastewater treatment system.Journal of environmental management · 2021Article
- Article
- Bacteria from the GenusApplied and environmental microbiology · 2020Article
- Exploring the antibiotic resistome in activated sludge and anaerobic digestion sludge in an urban wastewater treatment plant via metagenomic analysis.Journal of microbiology (Seoul, Korea) · 2020Article
- A comparative assessment of conventional and molecular methods, including MinION nanopore sequencing, for surveying water quality.Scientific reports · 2019Article
- Profiling Bacterial Diversity and Potential Pathogens in Wastewater Treatment Plants Using High-Throughput Sequencing Analysis.Microorganisms · 2019Article
- Reduction ofPathogens (Basel, Switzerland) · 2019Article
- Bacterial community structure in rotating biological contactor treating coke wastewater in relation to medium composition.Environmental science and pollution research international · 2019Article
- Is anaerobic digestion a reliable barrier for deactivation of pathogens in biosludge?The Science of the total environment · 2019Review
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study used 454 pyrosequencing, Illumina high-throughput sequencing and metagenomic analysis to investigate bacterial pathogens and their potential virulence in a sewage treatment plant (STP) applying both conventional and advanced treatment processes. Pyrosequencing and Illumina sequencing consistently demonstrated that Arcobacter genus occupied over 43.42% of total abundance of potential pathogens in the STP. At species level, potential pathogens Arcobacter butzleri, Aeromonas hydrophila and Klebsiella pneumonia dominated in raw sewage, which was also confirmed by quantitative real time PCR. Illumina sequencing also revealed prevalence of various types of pathogenicity islands and virulence proteins in the STP. Most of the potential pathogens and virulence factors were eliminated in the STP, and the removal efficiency mainly depended on oxidation ditch. Compared with sand filtration, magnetic resin seemed to have higher removals in most of the potential pathogens and virulence factors. However, presence of the residual A. butzleri in the final effluent still deserves more concerns. The findings indicate that sewage acts as an important source of environmental pathogens, but STPs can effectively control their spread in the environment. Joint use of the high-throughput sequencing technologies is considered a reliable method for deep and comprehensive overview of environmental bacterial virulence.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.