ArticlePloS one2015
A novel approach to identifying physical markers of cryo-damage in bull spermatozoa.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Integrated Proteomics and Metabolomics Reveal Spermine Enhances Sperm Freezability via Antioxidant Pathways.Antioxidants (Basel, Switzerland) · 2025Article
- Inexpensive thermal containers and insulation materials prevent deterioration of semen parameters for less than 90 minutes.The Journal of reproduction and development · 2025Article
- Impact of various cryo-preservation steps on sperm rheotaxis and sperm kinematics in bull.Scientific reports · 2024Article
- Exploring the full potential of sperm function with nanotechnology tools.Animal reproduction · 2024Article
- Molecular Markers: A New Paradigm in the Prediction of Sperm Freezability.International journal of molecular sciences · 2023Review
- Development of a biomolecular approach to identify sperm functions and fertility using sperm RNAs.Frontiers in cell and developmental biology · 2023Article
- Low Sperm Motility Is Determined by Abnormal Protein Modification during Epididymal Maturation.The world journal of men's health · 2022Article
- Heat shock protein family D member 1 in boar spermatozoa is strongly related to the litter size of inseminated sows.Journal of animal science and biotechnology · 2022Article
- Metabolomics analysis of buck semen cryopreserved with trehalose.Frontiers in genetics · 2022Article
- Evaluation of lipidomic change in goat sperm after cryopreservation.Frontiers in veterinary science · 2022Article
- Effect of glycerol addition time on the cryopreserved Korean native brindle cattle (Chikso) sperm quality.Animal reproduction · 2022Article
- Article
- Sperm Cryodamage in Ruminants: Understanding the Molecular Changes Induced by the Cryopreservation Process to Optimize Sperm Quality.International journal of molecular sciences · 2020Review
- Effect of Cooling and Freezing on Llama (Frontiers in veterinary science · 2020Article
- Freezability biomarkers in bull epididymal spermatozoa.Scientific reports · 2019Article
- Article
- Peroxiredoxin activity is a major landmark of male fertility.Scientific reports · 2017Article
- Seminal Plasma Heparin Binding Proteins Improve Semen Quality by Reducing Oxidative Stress during Cryopreservation of Cattle Bull Semen.Asian-Australasian journal of animal sciences · 2016Article
- A novel approach to assessing bisphenol-A hazards using an in vitro model system.BMC genomics · 2016Article
- In vitro capacitation and acrosome reaction in sperm of the phyllostomid bat Artibeus jamaicensis.In vitro cellular & developmental biology. Animal · 2016Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cryopreservation is an efficient way to store spermatozoa and plays a critical role in the livestock industry as well as in clinical practice. During cryopreservation, cryo-stress causes substantial damage to spermatozoa. In present study, the effects of cryo-stress at various cryopreservation steps, such as dilution / cooling, adding cryoprtectant, and freezing were studied in spermatozoa collected from 9 individual bull testes. The motility (%), motion kinematics, capacitation status, mitochondrial activity, and viability of bovine spermatozoa at each step of the cryopreservation process were assessed using computer-assisted sperm analysis, Hoechst 33258/chlortetracycline fluorescence, rhodamine 123 staining, and hypo-osmotic swelling test, respectively. The results demonstrate that the cryopreservation steps reduced motility (%), rapid speed (%), and mitochondrial activity, whereas medium/slow speed (%), and the acrosome reaction were increased (P < 0.05). Differences (Δ) of the acrosome reaction were higher in dilution/cooling step (P < 0.05), whereas differences (Δ) of motility, rapid speed, and non-progressive motility were higher in cryoprotectant and freezing as compared to dilution/cooling (P < 0.05). On the other hand, differences (Δ) of mitochondrial activity, viability, and progressive motility were higher in freezing step (P < 0.05) while the difference (Δ) of the acrosome reaction was higher in dilution/cooling (P < 0.05). Based on these results, we propose that freezing / thawing steps are the most critical in cryopreservation and may provide a logical ground of understanding on the cryo-damage. Moreover, these sperm parameters might be used as physical markers of sperm cryo-damage.
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