ArticleNature methods2015
Rapid, optimized interactomic screening.
Article in Nature methods, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed.
- O-Mannose Glycosylations Influence E-Cadherin Functional Interactions.Molecular & cellular proteomics : MCP · 2026Article
- Dominant negative mutations in yeast Hsp90 indicate triage decision mechanism targeting client proteins for degradation.Molecular biology of the cell · 2025Article
- Dominant negative mutations in yeast Hsp90 reveal triage decision mechanism targeting client proteins for degradation.bioRxiv : the preprint server for biology · 2024Article
- Multiparameter screen optimizes immunoprecipitation.BioTechniques · 2024Article
- CCDC88B interacts with RASAL3 and ARHGEF2 and regulates dendritic cell function in neuroinflammation and colitis.Communications biology · 2024Article
- Nuclear pore complexes mediate subtelomeric gene silencing by regulating PCNA levels on chromatin.The Journal of cell biology · 2023Article
- Proteomic elucidation of the targets and primary functions of the picornavirus 2A protease.The Journal of biological chemistry · 2022Article
- Near-atomic structure of the inner ring of the Saccharomyces cerevisiae nuclear pore complex.Cell research · 2022Article
- Characterizing Endogenous Protein Complexes with Biological Mass Spectrometry.Chemical reviews · 2022Review
- Affinity Isolation of Endogenous Saccharomyces Cerevisiae Nuclear Pore Complexes.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Chromatography-Free Purification Strategies for Large Biological Macromolecular Complexes Involving Fractionated PEG Precipitation and Density Gradients.Life (Basel, Switzerland) · 2021Review
- MeasuringSTAR protocols · 2021Article
- Heh2/Man1 may be an evolutionarily conserved sensor of NPC assembly state.Molecular biology of the cell · 2021Article
- Characterization of the RNA-Binding Protein TcSgn1 inMicroorganisms · 2021Article
- Dissecting the Structural Dynamics of the Nuclear Pore Complex.Molecular cell · 2021Article
- NCBP3 positively impacts mRNA biogenesis.Nucleic acids research · 2020Article
- Affinity proteomic dissection of the human nuclear cap-binding complex interactome.Nucleic acids research · 2020Article
- Proximity Dependent Biotinylation: Key Enzymes and Adaptation to Proteomics Approaches.Molecular & cellular proteomics : MCP · 2020Review
- Exocyst structural changes associated with activation of tethering downstream of Rho/Cdc42 GTPases.The Journal of cell biology · 2020Article
- Native Mass Spectrometry Analysis of Affinity-Captured Endogenous Yeast RNA Exosome Complexes.Methods in molecular biology (Clifton, N.J.) · 2020Article
Corrections and comments
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Authors and funding
15 authors.
Funding
Abstract
We must reliably map the interactomes of cellular macromolecular complexes in order to fully explore and understand biological systems. However, there are no methods to accurately predict how to capture a given macromolecular complex with its physiological binding partners. Here, we present a screening method that comprehensively explores the parameters affecting the stability of interactions in affinity-captured complexes, enabling the discovery of physiological binding partners in unparalleled detail. We have implemented this screen on several macromolecular complexes from a variety of organisms, revealing novel profiles for even well-studied proteins. Our approach is robust, economical and automatable, providing inroads to the rigorous, systematic dissection of cellular interactomes.
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