Evidence map›Paper›PMID 25937122›Full record

ArticleFEBS letters2015

Selective permeabilization of cervical cancer cells to an ionic DNA-binding cytotoxin by activation of P2Y receptors.

Maurish Bukhari, Han Deng, Noelle Jones, Zachary Towne, Craig D Woodworth, Damien S K Samways

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in FEBS letters, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.1field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 8 citations in OpenAlex.

  1. The P2 purinoceptors in prostate cancer.Purinergic signalling · 2023
    Review
  2. Review
  3. Review
  4. Purinergic Signalling: Therapeutic Developments.Frontiers in pharmacology · 2017
    Review
  5. KThe Journal of biological chemistry
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Maurish BukhariDepartment of Biology, Clarkson University, 8 Clarkson Ave., Potsdam, NY 13699-5805, USA.
Han DengDepartment of Biology, Clarkson University, 8 Clarkson Ave., Potsdam, NY 13699-5805, USA.
Noelle JonesDepartment of Biology, Clarkson University, 8 Clarkson Ave., Potsdam, NY 13699-5805, USA.
Zachary TowneDepartment of Biology, Clarkson University, 8 Clarkson Ave., Potsdam, NY 13699-5805, USA.
Craig D WoodworthDepartment of Biology, Clarkson University, 8 Clarkson Ave., Potsdam, NY 13699-5805, USA.
Damien S K SamwaysDepartment of Biology, Clarkson University, 8 Clarkson Ave., Potsdam, NY 13699-5805, USA. Electronic address: dsamways@clarkson.edu.
Clarkson University · US

Funding

Interaction of HPV with cells of the transformation zoneR15CA173703 · NCI · CLARKSON UNIVERSITY · PI WOODWORTH, CRAIG DUNCAN · 2013 to 2013
$436k
NCI NIH HHS 1R15CA173703-01NCI NIH HHS R15 CA173703
6 · The paper itself

Abstract

Extracellular ATP is known to permeabilize certain cell types to polyatomic cations like YO-PRO1. Here, we report that extracellularly applied ATP stimulated rapid uptake and accumulation of an otherwise weakly membrane permeable fluorescent DNA-binding cytotoxin, Hoechst 33258, into cervical cancer cells. While ATP stimulated Hoechst 33258 uptake in 20-70% of cells from seven cervical cancer cell lines, it stimulated uptake in less than 8% of cervical epithelial cells obtained from the normal transformation zone and ectocervix tissue of 11 patients. ATP-evoked Hoechst 33258 uptake was independent of ionotropic P2X receptors, but dependent on activation of P2Y receptors. Thus, we show here that cervical cancer cells can be selectively induced to take up and accumulate an ionic cytotoxin by exposure to extracellular ATP.

Indexed as

Cell Membrane PermeabilityAdenosine DiphosphateAdenosine TriphosphateBenzimidazolesBiological TransportCalciumCell Line, TumorCells, CulturedCytotoxinsDNA, NeoplasmEpithelial CellsFemaleHEK293 CellsHeLa CellsHumansMicroscopy, FluorescenceAdenosine DiphosphateAdenosine TriphosphateBenzimidazolesCalciumCytotoxinsDNA, Neoplasmhoechst 32258N(6)-methyl-2'-deoxyadenosine 3',5'-diphosphatePurinergic P2Y Receptor AgonistsPurinergic P2Y Receptor AntagonistsReceptors, Purinergic P2YUridine TriphosphateCa(2+)Cervical cancerDoxorubicinDrug deliveryHoechst 33258P2XP2YPermeabilityPibenzimolPurinergic

Identifiers

PMID25937122
PMCPMC4497545
OpenAlexW2140934991

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.