Evidence map›Paper›PMID 25936875›Full record

ArticleExperimental neurology2015

MFGE8/Integrin β3 pathway alleviates apoptosis and inflammation in early brain injury after subarachnoid hemorrhage in rats.

Fei Liu, Yujie Chen, Qin Hu, Bo Li, Junjia Tang, Yue He, Zongduo Guo, Hua Feng, Jiping Tang, John H Zhang

RetractedOpen access · greenAbstract readRetracted Publication
PubMed Full text at PMC Publisher
In one paragraph

Article in Experimental neurology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 29 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
29citing papers in PubMed, 1 pooled it
1.7field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

29 citing papers in PubMed, 1 synthesis or guideline pooled it, 49 citations in OpenAlex.

  1. Pooled it
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  13. EGCG Promotes Neurite Outgrowth through the IntegrinEvidence-based complementary and alternative medicine : eCAM · 2021
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 2 countries.

Fei LiuDepartment of Neurosurgery, The Third Xiangya Hospital, Central South University, Changsha, Hunan, China; Department of Anesthesiology, Loma Linda University, CA, USA; Department of Neurosurgery, Loma Linda University, CA, USA; Department of Physiology, Loma Linda University, CA, USA.
Yujie ChenDepartment of Neurosurgery, Southwest Hospital, Third Military Medical University, Chongqing, China; Department of Anesthesiology, Loma Linda University, CA, USA; Department of Neurosurgery, Loma Linda University, CA, USA; Department of Physiology, Loma Linda University, CA, USA.
Qin HuDepartment of Anesthesiology, Loma Linda University, CA, USA; Department of Neurosurgery, Loma Linda University, CA, USA; Department of Physiology, Loma Linda University, CA, USA.
Bo LiDepartment of Anesthesiology, Loma Linda University, CA, USA; Department of Neurosurgery, Loma Linda University, CA, USA; Department of Physiology, Loma Linda University, CA, USA.
Junjia TangDepartment of Anesthesiology, Loma Linda University, CA, USA; Department of Neurosurgery, Loma Linda University, CA, USA; Department of Physiology, Loma Linda University, CA, USA.
Yue HeDepartment of Anesthesiology, Loma Linda University, CA, USA; Department of Neurosurgery, Loma Linda University, CA, USA; Department of Physiology, Loma Linda University, CA, USA.
Zongduo GuoDepartment of Anesthesiology, Loma Linda University, CA, USA; Department of Neurosurgery, Loma Linda University, CA, USA; Department of Physiology, Loma Linda University, CA, USA.
Hua FengDepartment of Neurosurgery, Southwest Hospital, Third Military Medical University, Chongqing, China.
Jiping TangDepartment of Anesthesiology, Loma Linda University, CA, USA; Department of Neurosurgery, Loma Linda University, CA, USA; Department of Physiology, Loma Linda University, CA, USA.
John H ZhangDepartment of Anesthesiology, Loma Linda University, CA, USA; Department of Neurosurgery, Loma Linda University, CA, USA; Department of Physiology, Loma Linda University, CA, USA. Electronic address: johnzhang3910@yahoo.com.
Loma Linda University · USArmy Medical University · CNCentral South University · CNLoma Linda University Medical Center · US

Funding

Vascular Biology Core FacilityP01NS082184 · NINDS · LOMA LINDA UNIVERSITY · PI PEARCE, WILLIAM J. · 2014 to 2018
$6.1M
Neurovascular Protection for Early Brain Injury after SAHR01NS081740 · NINDS · LOMA LINDA UNIVERSITY · PI ZHANG, JOHN H · 2013 to 2017
$1.6M
NINDS NIH HHS NS081740NINDS NIH HHS NS082184NINDS NIH HHS P01 NS082184NINDS NIH HHS R01 NS081740
6 · The paper itself

Abstract

backgroundMilk fat globule-epidermal growth factor-factor 8(MFGE8)/Integrin β3 pathway was reported to be involved in reducing oxidative stress and early brain injury after subarachnoid hemorrhage (SAH). In the present study, the potential effects of MFGE8 and its receptor Integrin β3 in the inhibition of apoptosis and neuroinflammation in early brain injury after SAH were investigated.

methodsNinety-five (95) male Sprague-Dawley rats were used. The SAH model was induced by endovascular perforation. Recombinant human MFGE8 (rhMFGE8), MFGE8 small interfering RNA (siRNA) and Integrin β3 siRNA were injected intracerebroventricularly. SAH grade, neurologic scores, Western blots and immunofluorescence were employed to study the mechanisms of MFGE8 and its receptor Integrin β3, as well as neurological outcome.

resultsSAH induced significant neuronal apoptosis and inflammation and exhibited neurological dysfunction in rats. Knockdown endogenous MFGE8 with siRNA significantly increased the protein levels of cleaved caspase 3 and IL-1β, accompanied with more neurological deficits. rhMFGE8 significantly reduced neural cell death in cortex, decreased cleaved caspase 3 and IL-1β expressions, and improved neurological functions 24h after SAH. The anti-apoptosis and anti-inflammation effects of rhMFGE8 were abolished by Integrin β3 siRNA.

conclusionMFGE8 could alleviate neurologic damage in early brain injury after SAH via anti-inflammation and anti-apoptosis effects. MFGE8 may serve as a promising therapeutic target for future management of SAH patients.

Indexed as

AnimalsAntigens, SurfaceApoptosisBrain InjuriesCaspase 3Disease Models, AnimalEncephalitisGene Expression RegulationInfusions, IntraventricularIntegrin beta3Interleukin-1betaLocomotionMaleMilk ProteinsPhosphopyruvate HydratasePsychomotor PerformanceAntigens, SurfaceCaspase 3IL1B protein, ratIntegrin beta3Interleukin-1betaMFGE8 protein, ratMilk ProteinsPhosphopyruvate HydrataseRNA, Small InterferingApoptosisEarly brain injuryInflammationMFGE8Subarachnoid hemorrhage

Identifiers

PMID25936875
PMCPMC4628907
OpenAlexW1973207697

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.