Evidence map›Paper›PMID 25936696›Full record

ArticleNeuroImage2015

Direct in vivo assessment of human stem cell graft-host neural circuits.

Blake Byers, Hyun Joo Lee, Jia Liu, Andrew J Weitz, Peter Lin, Pengbo Zhang, Aleksandr Shcheglovitov, Ricardo Dolmetsch, Renee Reijo Pera, Jin Hyung Lee

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in NeuroImage, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
2.4field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 36 citations in OpenAlex.

  1. Bioengineering & translational medicine · 2024
    Article
  2. Towards translational optogenetics.Nature biomedical engineering · 2023
    Review
  3. Review
  4. Review
  5. Article
  6. Review
  7. Optogenetic Interrogation of Circuits Following Neurotrauma.Frontiers in molecular neuroscience · 2021
    Review
  8. Application of Optogenetics for Muscle Cells and Stem Cells.Advances in experimental medicine and biology · 2021
    Article
  9. Review
  10. Article
  11. Review
  12. The Journal of neuroscience : the official journal of the Society for Neuroscience · 2017
    Review
  13. Review
  14. Article
  15. Review
  16. Optogenetic Functional MRI.Journal of visualized experiments : JoVE · 2016
    Article
  17. Review
  18. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 1 country.

Blake ByersDepartment of Bioengineering, Stanford University, CA 94305, USA; Institute for Stem Cell Biology and Regenerative Medicine, Stanford University, CA 94305, USA; Google Ventures, Mountain View, CA 94043, USA.
Hyun Joo LeeDepartment of Neurology and Neurological Sciences, Stanford University, CA 94305, USA.
Jia LiuDepartment of Neurology and Neurological Sciences, Stanford University, CA 94305, USA; Department of Psychiatry and Biobehavioral Sciences, Neuroscience Interdepartmental Program, University of California, Los Angeles, CA 90095, USA.
Andrew J WeitzDepartment of Bioengineering, Stanford University, CA 94305, USA.
Peter LinDepartment of Neurology and Neurological Sciences, Stanford University, CA 94305, USA.
Pengbo ZhangInstitute for Stem Cell Biology and Regenerative Medicine, Stanford University, CA 94305, USA.
Aleksandr ShcheglovitovDepartment of Neurobiology and Anatomy, University of Utah, Salt Lake City, UT 84132, USA; Department of Neurobiology, Stanford University, CA 94305, USA.
Ricardo DolmetschDepartment of Neurobiology, Stanford University, CA 94305, USA.
Renee Reijo PeraInstitute for Stem Cell Biology and Regenerative Medicine, Stanford University, CA 94305, USA; Department of Obstetrics and Gynecology, Stanford University, CA 94305, USA.
Jin Hyung LeeDepartment of Bioengineering, Stanford University, CA 94305, USA; Department of Neurology and Neurological Sciences, Stanford University, CA 94305, USA; Department of Psychiatry and Biobehavioral Sciences, Neuroscience Interdepartmental Program, University of California, Los Angeles, CA 90095, USA; Department of Neurosurgery, Stanford University, CA 94305, USA; Department of Electrical Engineering, Stanford University, CA 94305, USA. Electronic address: ljinhy@stanford.edu.
Stanford University · USCalifornia Institute for Regenerative Medicine · USNeurobehavioral Systems · US

Funding

Using induced pluripotent stem cells to identify cellular phenotypes of autismDP1OD003889 · OD · STANFORD UNIVERSITY · PI DOLMETSCH, RICARDO E. · 2008 to 2011
$3.2M
In Vivo Control and Functional Visualization of Stem Cell-Driven CNS RegenerationDP2OD007265 · OD · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI LEE, JIN HYUNG · 2010 to 2010
$2.4M
Visualization of Neuro-Molecular Targeting using Distribution-Free, High-Res fMRIR00EB008738 · NIBIB · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI LEE, JIN HYUNG · 2010 to 2012
$745k
NIBIB NIH HHS 4R00EB008738NIBIB NIH HHS R00 EB008738NIH HHS 1DP2OD007265NIH HHS DP1 OD003889NIH HHS DP2 OD007265
6 · The paper itself

Abstract

Despite the potential of stem cell-derived neural transplants for treating intractable neurological diseases, the global effects of a transplant's electrical activity on host circuitry have never been measured directly, preventing the systematic optimization of such therapies. Here, we overcome this problem by combining optogenetics, stem cell biology, and neuroimaging to directly map stem cell-driven neural circuit formation in vivo. We engineered human induced pluripotent stem cells (iPSCs) to express channelrhodopsin-2 and transplanted resulting neurons to striatum of rats. To non-invasively visualize the function of newly formed circuits, we performed high-field functional magnetic resonance imaging (fMRI) during selective stimulation of transplanted cells. fMRI successfully detected local and remote neural activity, enabling the global graft-host neural circuit function to be assessed. These results demonstrate the potential of a novel neuroimaging-based platform that can be used to identify how a graft's electrical activity influences the brain network in vivo.

Indexed as

AnimalsBrainBrain MappingCorpus StriatumEmbryonic Stem CellsFemaleHeterograftsHumansInduced Pluripotent Stem CellsMagnetic Resonance ImagingOptogeneticsRatsFunctional MRIOptogeneticsStem cellTransplant

Identifiers

PMID25936696
PMCPMC5573170
OpenAlexW1998884570

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.