ArticleJournal of microbiology (Seoul, Korea)2015
Identification of seven novel virulence genes from Xanthomonas citri subsp. citri by Tn5-based random mutagenesis.
Article in Journal of microbiology (Seoul, Korea), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 24 citations in OpenAlex.
- Xanthomonas citri subsp. citri requires a polyketide cyclase to activate the type III secretion system for virulence.BMC microbiology · 2025Article
- Article
- Review
- The Methyltransferase HemK Regulates the Virulence and Nutrient Utilization of the Phytopathogenic BacteriumInternational journal of molecular sciences · 2022Article
- ThePathogens (Basel, Switzerland) · 2021Article
- An inducible transposon mutagenesis approach for the intracellular human pathogenWellcome open research · 2021Article
- Identification of Essential Genes Associated With Prodigiosin Production inFrontiers in microbiology · 2021Article
- Tn5 Transposase Applied in Genomics Research.International journal of molecular sciences · 2020Review
- Inhibition of the Citrus Canker Pathogen Using a Photosensitizer Assisted by Sunlight Irradiation.Frontiers in microbiology · 2020Article
- Global Regulator PhoP is Necessary for Motility, Biofilm Formation, Exoenzyme Production and Virulence ofGenes · 2019Article
- A practical random mutagenesis system for Ralstonia solanacearum strains causing bacterial wilt of Pogostemon cablin using Tn5 transposon.World journal of microbiology & biotechnology · 2018Article
- TheFrontiers in microbiology · 2018Article
- Salmonella Persistence in Tomatoes Requires a Distinct Set of Metabolic Functions Identified by Transposon Insertion Sequencing.Applied and environmental microbiology · 2017Article
- Identification of an Extracellular Endoglucanase That Is Required for Full Virulence in Xanthomonas citri subsp. citri.PloS one · 2016Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To identify novel virulence genes, a mutant library of Xanthomonas citri subsp. citri 29-1 was produced using EZ-Tn5 transposon and the mutants were inoculated into susceptible grapefruit. Forty mutants with altered virulence phenotypes were identified. Nine of the mutants showed a complete loss of citrus canker induction, and the other 31 mutants resulted in attenuated canker symptoms. Southern blot analysis revealed that each of the mutants carried a single copy of Tn5. The flanking sequence was identified by plasmid rescue and 18 different ORFs were identified in the genome sequence. Of these 18 ORFs, seven had not been previously associated with the virulence of X. citri subsp. citri and were therefore confirmed by complementation analysis. Real-time PCR analysis showed that the seven genes were upregulated when the bacteria were grown in citrus plants, suggesting that the expression of these genes was essential for canker development.
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