Evidence map›Paper›PMID 25935260›Full record

ArticleAnalytical biochemistry2015

Sensing pH via p-cyanophenylalanine fluorescence: Application to determine peptide pKa and membrane penetration kinetics.

Ileana M Pazos, Ismail A Ahmed, Mariana I León Berríos, Feng Gai

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Analytical biochemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.1field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 14 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
  4. Minimalist IR and fluorescence probes of protein function.Current opinion in chemical biology · 2016
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 2 countries.

Ileana M PazosDepartment of Chemistry, University of Pennsylvania, Philadelphia, PA 19104, USA.
Ismail A AhmedDepartment of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, PA 19104, USA.
Mariana I León BerríosDepartment of Chemistry, University of Puerto Rico at Cayey, Cayey 00736, Puerto Rico.
Feng GaiDepartment of Chemistry, University of Pennsylvania, Philadelphia, PA 19104, USA. Electronic address: gai@sas.upenn.edu.
University of Pennsylvania · USUniversity of Puerto Rico at Cayey · PR

Funding

ULTRAFAST OPTICAL PROCESSES LABORATORYP41GM104605 · NIGMS · UNIVERSITY OF PENNSYLVANIA · PI GAI, FENG · 2012 to 2017
$5.6M
Spectroscopic Study of Protein Folding DynamicsR01GM065978 · NIGMS · UNIVERSITY OF PENNSYLVANIA · PI GAI, FENG · 2002 to 2014
$3.9M
NIGMS NIH HHS GM-065978NIGMS NIH HHS P41 GM104605NIGMS NIH HHS R01 GM065978
6 · The paper itself

Abstract

We expand the spectroscopic utility of a well-known infrared and fluorescence probe, p-cyanophenylalanine, by showing that it can also serve as a pH sensor. This new application is based on the notion that the fluorescence quantum yield of this unnatural amino acid, when placed at or near the N-terminal end of a polypeptide, depends on the protonation status of the N-terminal amino group of the peptide. Using this pH sensor, we are able to determine the N-terminal pKa values of nine tripeptides and also the membrane penetration kinetics of a cell-penetrating peptide. Taken together, these examples demonstrate the applicability of using this unnatural amino acid fluorophore to study pH-dependent biological processes or events that accompany a pH change.

Indexed as

AlanineAminesCell MembraneCell-Penetrating PeptidesHydrogen-Ion ConcentrationKineticsNitrilesPhenylalanineSpectrometry, FluorescenceAlanineAminesCell-Penetrating PeptidesNitrilesp-cyanophenylalaninePhenylalanineCell-penetrating peptideFluorescence spectroscopyN-terminal pK(a) of peptidep-CyanophenylalaninepH sensor

Identifiers

PMID25935260
PMCPMC4461448
OpenAlexW2092471697

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.