ArticleJournal of cell science2015
Dgcr8 and Dicer are essential for sex chromosome integrity during meiosis in males.
Article in Journal of cell science, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 49 citations in OpenAlex.
- Small Non-coding RNAs and Male Infertility.Advances in experimental medicine and biology · 2026Review
- A TOPBP1 allele causing male infertility uncouples XY silencing dynamics from sex body formation.eLife · 2024Article
- MicroRNAs in spermatogenesis dysfunction and male infertility: clinical phenotypes, mechanisms and potential diagnostic biomarkers.Frontiers in endocrinology · 2024Review
- Review
- Chromatin remodelers HELLS, WDHD1 and BAZ1A are dynamically expressed during mouse spermatogenesis.Reproduction (Cambridge, England) · 2023Article
- MicroRNA-202 safeguards meiotic progression by preventing premature SEPARASE-mediated REC8 cleavage.EMBO reports · 2022Article
- Regulation of mammalian spermatogenesis by miRNAs.Seminars in cell & developmental biology · 2022Review
- Small Noncoding RNAs in Reproduction and Infertility.Biomedicines · 2021Review
- CRL4-DCAF12 Ubiquitin Ligase Controls MOV10 RNA Helicase during Spermatogenesis and T Cell Activation.International journal of molecular sciences · 2021Article
- The Epigenetics of Gametes and Early Embryos and Potential Long-Range Consequences in Livestock Species-Filling in the Picture With Epigenomic Analyses.Frontiers in genetics · 2021Review
- Regulation of Meiotic Prophase One in Mammalian Oocytes.Frontiers in cell and developmental biology · 2021Review
- RNAi-Related Dicer and Argonaute Proteins Play Critical Roles for Meiocyte Formation, Chromosome-Axes Lengths and Crossover Patterning in the FungusFrontiers in cell and developmental biology · 2021Article
- Regulation of Human Spermatogenesis.Advances in experimental medicine and biology · 2021Article
- The Role of Small Noncoding RNA in DNA Double-Strand Break Repair.International journal of molecular sciences · 2020Review
- From the Argonauts Mythological Sailors to the Argonautes RNA-Silencing Navigators: Their Emerging Roles in Human-Cell Pathologies.International journal of molecular sciences · 2020Review
- Deficient Expression ofInternational journal of general medicine · 2020Article
- Pharmacological boost of DNA damage response and repair by enhanced biogenesis of DNA damage response RNAs.Scientific reports · 2019Article
- Roles of microRNAs in mammalian reproduction: from the commitment of germ cells to peri-implantation embryos.Biological reviews of the Cambridge Philosophical Society · 2019Review
- Dynamic reorganization of the genome shapes the recombination landscape in meiotic prophase.Nature structural & molecular biology · 2019Article
- Genes involved in miRNA biogenesis affect meiosis and fertility.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2018Review
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
Small RNAs play crucial roles in regulating gene expression during mammalian meiosis. To investigate the function of microRNAs (miRNAs) and small interfering RNAs (siRNAs) during meiosis in males, we generated germ-cell-specific conditional deletions of Dgcr8 and Dicer in mice. Analysis of spermatocytes from both conditional knockout lines revealed that there were frequent chromosomal fusions during meiosis, always involving one or both sex chromosomes. RNA sequencing indicates upregulation of Atm in spermatocytes from miRNA-deficient mice, and immunofluorescence imaging demonstrates an increased abundance of activated ATM kinase and mislocalization of phosphorylated MDC1, an ATM phosphorylation substrate. The Atm 3'UTR contains many potential microRNA target sites, and, notably, target sites for several miRNAs depleted in both conditional knockout mice were highly effective at promoting repression. RNF8, a telomere-associated protein whose localization is controlled by the MDC1-ATM kinase cascade, normally associates with the sex chromosomes during pachytene, but in both conditional knockouts redistributed to the autosomes. Taken together, these results suggest that Atm dysregulation in microRNA-deficient germ lines contributes to the redistribution of proteins involved in chromosomal stability from the sex chromosomes to the autosomes, resulting in sex chromosome fusions during meiotic prophase I.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.