ArticleEukaryotic cell2015
Chitinases Are Essential for Cell Separation in Ustilago maydis.
Article in Eukaryotic cell, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 59 citations in OpenAlex.
- The Ratio of Silicon to Chitin in Diatom Cell Walls Influences Their Resilience to Environmental Stress.Research (Washington, D.C.) · 2026Article
- α-glucan remodeling by GH13-domain enzymes shapes fungal cell wall architecture.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- TheApplied and environmental microbiology · 2023Article
- Plant immunity suppression by an exo-β-1,3-glucanase and an elongation factor 1α of the rice blast fungus.Nature communications · 2023Article
- An overview of fungal chitinases and their potential applications.Protoplasma · 2023Review
- Silencing a Chitinase Gene,International journal of molecular sciences · 2023Article
- Article
- Review
- Controlling Unconventional Secretion for Production of Heterologous Proteins inJournal of fungi (Basel, Switzerland) · 2021Article
- Genetic Manipulation of the Brassicaceae Smut FungusJournal of fungi (Basel, Switzerland) · 2021Article
- A Novel Potent Carrier for Unconventional Protein Export inFrontiers in cell and developmental biology · 2021Article
- Molecular Mechanisms Involved in the Multicellular Growth of Ustilaginomycetes.Microorganisms · 2020Review
- A Novel Factor Essential for Unconventional Secretion of Chitinase Cts1.Frontiers in microbiology · 2020Article
- Microbial chitinases: properties, current state and biotechnological applications.World journal of microbiology & biotechnology · 2019Review
- Chitinases Play a Key Role in Stipe Cell Wall Extension in the MushroomApplied and environmental microbiology · 2019Article
- APlant physiology · 2019Article
- A Potential Lock-Type Mechanism for Unconventional Secretion in Fungi.International journal of molecular sciences · 2019Review
- Binding of theThe Plant cell · 2019Article
- Applying Unconventional Secretion in Ustilago maydis for the Export of Functional Nanobodies.International journal of molecular sciences · 2017Article
- The ESCRT regulator Did2 maintains the balance between long-distance endosomal transport and endocytic trafficking.PLoS genetics · 2017Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chitin is an essential component of the fungal cell wall, providing rigidity and stability. Its degradation is mediated by chitinases and supposedly ensures the dynamic plasticity of the cell wall during growth and morphogenesis. Hence, chitinases should be particularly important for fungi with dramatic morphological changes, such as Ustilago maydis. This smut fungus switches from yeast to filamentous growth for plant infection, proliferates as a mycelium in planta, and forms teliospores for spreading. Here, we investigate the contribution of its four chitinolytic enzymes to the different morphological changes during the complete life cycle in a comprehensive study of deletion strains combined with biochemical and cell biological approaches. Interestingly, two chitinases act redundantly in cell separation during yeast growth. They mediate the degradation of remnant chitin in the fragmentation zone between mother and daughter cell. In contrast, even the complete lack of chitinolytic activity does not affect formation of the infectious filament, infection, biotrophic growth, or teliospore germination. Thus, unexpectedly we can exclude a major role for chitinolytic enzymes in morphogenesis or pathogenicity of U. maydis. Nevertheless, redundant activity of even two chitinases is essential for cell separation during saprophytic growth, possibly to improve nutrient access or spreading of yeast cells by wind or rain.
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