Evidence map›Paper›PMID 25934662›Full record

ArticleThe Journal of nutrition2015

Maternal protein restriction increases respiratory and sympathetic activities and sensitizes peripheral chemoreflex in male rat offspring.

José L de Brito Alves, Viviane O Nogueira, Marinaldo P Cavalcanti Neto, Andréia M Leopoldino, Carlos Curti, Débora S A Colombari, Eduardo Colombari, Almir G Wanderley, Carol G Leandro, Daniel B Zoccal and 1 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of nutrition, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
9.8field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 38 citations in OpenAlex.

  1. Review
  2. Prenatal LPS leads to increases in RAS expression within the PVN and overactivation of sympathetic outflow in offspring rats.Hypertension research : official journal of the Japanese Society of Hypertension · 2024
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  10. Carotid body removal normalizes arterial blood pressure and respiratory frequency in offspring of protein-restricted mothers.Hypertension research : official journal of the Japanese Society of Hypertension · 2018
    Article
  11. Review
  12. Low-protein diet does not alter reproductive, biochemical, and hematological parameters in pregnant Wistar rats.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2018
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 1 country.

José L de Brito AlvesDepartment of Physical Education and Sport Sciences, Federal University of Pernambuco, Vitoria de Santo Antão, Pernambuco, Brazil;
Viviane O NogueiraDepartment of Physical Education and Sport Sciences, Federal University of Pernambuco, Vitoria de Santo Antão, Pernambuco, Brazil;
Marinaldo P Cavalcanti NetoDepartments of Physics and Chemistry and Clinical Analyses, Toxicology and Food Sciences, School of Pharmaceutical Sciences, University of São Paulo, Ribeirão Preto, São Paulo, Brazil;
Andréia M LeopoldinoDepartments of Physics and Chemistry and Clinical Analyses, Toxicology and Food Sciences, School of Pharmaceutical Sciences, University of São Paulo, Ribeirão Preto, São Paulo, Brazil;
Carlos CurtiDepartments of Physics and Chemistry and Clinical Analyses, Toxicology and Food Sciences, School of Pharmaceutical Sciences, University of São Paulo, Ribeirão Preto, São Paulo, Brazil;
Débora S A ColombariDepartment of Physiology and Pathology, School of Dentistry of Araraquara, São Paulo State University, Araraquara, São Paulo, Brazil; and.
Eduardo ColombariDepartment of Physiology and Pathology, School of Dentistry of Araraquara, São Paulo State University, Araraquara, São Paulo, Brazil; and.
Almir G WanderleyDepartment of Physiology and Pharmacology, Federal University of Pernambuco, Pernambuco, Brazil.
Carol G LeandroDepartment of Physical Education and Sport Sciences, Federal University of Pernambuco, Vitoria de Santo Antão, Pernambuco, Brazil;
Daniel B ZoccalDepartment of Physiology and Pharmacology, Federal University of Pernambuco, Pernambuco, Brazil.
João H Costa-SilvaDepartment of Physical Education and Sport Sciences, Federal University of Pernambuco, Vitoria de Santo Antão, Pernambuco, Brazil; joao.hcsilva@ufpe.br.
Universidade Federal de Pernambuco · BRUniversidade de São Paulo · BRCentro Universitário de Araraquara · BR

Funding

CRCNS: Modeling the respiratory-sympathetic coupling in neurogenic hypertensionR01AT008632 · NCCIH · INDIANA UNIVERSITY INDIANAPOLIS · PI MOLKOV, YAROSLAV · 2015 to 2019
$1.4M
NCCIH NIH HHS R01 AT008632
6 · The paper itself

Abstract

backgroundMaternal protein restriction in rats increases the risk of adult offspring arterial hypertension through unknown mechanisms.

objectivesThe aims of the study were to evaluate the effects of a low-protein (LP) diet during pregnancy and lactation on baseline sympathetic and respiratory activities and peripheral chemoreflex sensitivity in the rat offspring.

methodsWistar rat dams were fed a control [normal-protein (NP); 17% protein] or an LP (8% protein) diet during pregnancy and lactation, and their male offspring were studied at 30 d of age. Direct measurements of baseline arterial blood pressure (ABP), heart rate (HR), and respiratory frequency (Rf) as well as peripheral chemoreflex activation (potassium cyanide: 0.04%) were recorded in pups while they were awake. In addition, recordings of the phrenic nerve (PN) and thoracic sympathetic nerve (tSN) activities were obtained from the in situ preparations. Hypoxia-inducible factor 1α (HIF-1α) expression was also evaluated in carotid bifurcation through a Western blotting assay.

resultsAt 30 d of age, unanesthetized LP rats exhibited enhanced resting Rf (P = 0.001) and similar ABP and HR compared with the NP rats. Despite their similar baseline ABP values, LP rats exhibited augmented low-frequency variability (∼91%; P = 0.01). In addition, the unanesthetized LP rats showed enhanced pressor (P = 0.01) and tachypnoeic (P = 0.03) responses to peripheral chemoreflex activation. The LP rats displayed elevated baseline tSN activity (∼86%; P = 0.02) and PN burst frequency (45%; P = 0.01) and amplitude (53%; P = 0.001) as well as augmented sympathetic (P = 0.01) and phrenic (P = 0.04) excitatory responses to peripheral chemoreflex activation compared with the NP group. Furthermore, LP rats showed an increase of ∼100% in HIF-1α protein density in carotid bifurcation compared with NP rats.

conclusionSympathetic-respiratory overactivity and amplified peripheral chemoreceptor responses, potentially through HIF-1α-dependent mechanisms, precede the onset of hypertension in juvenile rats exposed to protein undernutrition during gestation and lactation.

Indexed as

Maternal Nutritional Physiological PhenomenaAnimalsBirth WeightCarotid Artery, CommonChemoreceptor CellsDiet, Protein-RestrictedFemaleFetal DevelopmentFetal Growth RetardationHypoxia-Inducible Factor 1, alpha SubunitLactationMalePeripheral Nervous SystemPhrenic NervePregnancyPrehypertensionHif1a protein, ratHypoxia-Inducible Factor 1, alpha Subunithypertensionhypoxia-inducible factor 1 αperipheral chemoreflexprotein undernutritionsympathetic overactivity

Identifiers

PMID25934662
PMCPMC6619683
OpenAlexW2118954133

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.