ArticleApplied and environmental microbiology2015
Extracellular Vesicles of the Hyperthermophilic Archaeon "Thermococcus onnurineus" NA1T.
Article in Applied and environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 37 citations in OpenAlex.
- Unveiling Marine Vesicle Uptake in Vibrio spp: Taxonomic and Environmental Insights.MicrobiologyOpen · 2026Article
- Biogenesis of DNA-carrying extracellular vesicles by the dominant human gut methanogenic archaeon.Nature communications · 2025Article
- Extracellular Vesicles in Bacteria, Archaea, and Eukaryotes: Mechanisms of Inter-Kingdom Communication and Clinical Implications.Microorganisms · 2025Review
- Secreted nucleases reclaim extracellular DNA during biofilm development.NPJ biofilms and microbiomes · 2024Article
- Extracellular vesicle formation inProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Exploring the Archaeal Virosphere by Metagenomics.Methods in molecular biology (Clifton, N.J.) · 2024Article
- The secretome of the fish pathogenFrontiers in cellular and infection microbiology · 2023Article
- Translocated Legionella pneumophila small RNAs mimic eukaryotic microRNAs targeting the host immune response.Nature communications · 2022Article
- Isolation, Purification, and Characterization of Membrane Vesicles from Haloarchaea.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Biogenesis and Biological Functions of Extracellular Vesicles in Cellular and Organismal Communication With Microbes.Frontiers in microbiology · 2022Review
- Archaeal extracellular vesicles are produced in an ESCRT-dependent manner and promote gene transfer and nutrient cycling in extreme environments.The ISME journal · 2021Article
- Extracellular membrane vesicles and nanotubes in Archaea.microLife · 2021Review
- Extracellular tRNAs and tRNA-derived fragments.RNA biology · 2020Review
- Thermococcus indicus sp. nov., a Fe(III)-reducing hyperthermophilic archaeon isolated from the Onnuri Vent Field of the Central Indian Ocean ridge.Journal of microbiology (Seoul, Korea) · 2020Article
- Gut microbiota: a new player in regulating immune- and chemo-therapy efficacy.Cancer drug resistance (Alhambra, Calif.) · 2020Article
- Production of extracellular vesicles with light-induced proton pump activity by proteorhodopsin-containing marine bacteria.MicrobiologyOpen · 2019Article
- Extracellular membrane vesicles in the three domains of life and beyond.FEMS microbiology reviews · 2019Review
- Comparative Genomics of the GenusFrontiers in microbiology · 2019Article
- Candida albicans biofilm-induced vesicles confer drug resistance through matrix biogenesis.PLoS biology · 2018Article
- Mechanisms of gene flow in archaea.Nature reviews. Microbiology · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Extracellular vesicles (EVs) produced by a sulfur-reducing, hyperthermophilic archaeon, "Thermococcus onnurineus" NA1(T), were purified and characterized. A maximum of four EV bands, showing buoyant densities between 1.1899 and 1.2828 g cm(-3), were observed after CsCl ultracentrifugation. The two major EV bands, B (buoyant density at 25°C [ρ(25)] = 1.2434 g cm(-3)) and C (ρ(25) = 1.2648 g cm(-3)), were separately purified and counted using a qNano particle analyzer. These EVs, showing different buoyant densities, were identically spherical in shape, and their sizes varied from 80 to 210 nm in diameter, with 120- and 190-nm sizes predominant. The average size of DNA packaged into EVs was about 14 kb. The DNA of the EVs in band C was sequenced and assembled. Mapping of the T. onnurineus NA1(T) EV (ToEV) DNA sequences onto the reference genome of the parent archaeon revealed that most genes of T. onnurineus NA1(T) were packaged into EVs, except for an ∼9.4-kb region from TON_0536 to TON_0544. The absence of this specific region of the genome in the EVs was confirmed from band B of the same culture and from bands B and C purified from a different batch culture. The presence of the 3'-terminal sequence and the absence of the 5'-terminal sequence of TON_0536 were repeatedly confirmed. On the basis of these results, we hypothesize that the unpackaged part of the T. onnurineus NA1(T) genome might be related to the process that delivers DNA into ToEVs and/or the mechanism generating the ToEVs themselves.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.