Evidence map›Paper›PMID 25934457›Full record

ArticleBiomaterials2015

Engineered heart slices for electrophysiological and contractile studies.

Adriana Blazeski, Geran M Kostecki, Leslie Tung

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomaterials, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
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  16. Engineering cardiac microphysiological systems to model pathological extracellular matrix remodeling.American journal of physiology. Heart and circulatory physiology · 2018
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Adriana BlazeskiDepartment of Biomedical Engineering, The Johns Hopkins University, Baltimore, MD, USA.
Geran M KosteckiDepartment of Biomedical Engineering, The Johns Hopkins University, Baltimore, MD, USA.
Leslie TungDepartment of Biomedical Engineering, The Johns Hopkins University, Baltimore, MD, USA. Electronic address: ltung@jhu.edu.

Funding

Request for Zeiss LSM 510 MP Multiphoton Imaging SystemS10RR024550 · NCRR · JOHNS HOPKINS UNIVERSITY · PI KUO, SCOT CHARLES · 2008 to 2008
$500k
JHU Ultima-L Imaging SystemS10RR025544 · NCRR · JOHNS HOPKINS UNIVERSITY · PI TUNG, LESLIE · 2009 to 2009
$158k
NCRR NIH HHS S10 RR024550NCRR NIH HHS S10 RR025544
6 · The paper itself

Abstract

A major consideration in the design of engineered cardiac tissues for the faithful representation of physiological behavior is the recapitulation of the complex topography and biochemistry of native tissue. In this study we present engineered heart slices (EHS), which consist of neonatal rat ventricular cells (NRVCs) seeded onto thin slices of decellularized cardiac tissue that retain important aspects of native extracellular matrix (ECM). To form EHS, rat or pig ventricular tissue was sectioned into 300 μm-thick, 5 to 16 mm-diameter disks, which were subsequently decellularized using detergents, spread on coverslips, and seeded with NRVCs. The organized fiber structure of the ECM remained after decellularization and promoted cell elongation and alignment, resulting in an anisotropic, functional tissue that could be electrically paced. Contraction decreased at higher pacing rates, and optical mapping revealed electrical conduction that was anisotropic with a ratio of approximately 2.0, rate-dependent shortening of the action potential and slowing of conduction, and slowing of conduction by the sodium channel blocker lidocaine. Reentrant arrhythmias could also be pace-induced and terminated. EHS constitute an attractive in vitro cardiac tissue in which cardiac cells are cultured on thin slices of decellularized cardiac ECM that provide important biochemical, structural, and mechanical cues absent in traditional cell cultures.

Indexed as

Myocardial ContractionAnimalsAnisotropyArrhythmias, CardiacCells, CulturedDetergentsElectrophysiological PhenomenaExtracellular MatrixHeartHeart VentriclesIn Vitro TechniquesLidocaineMyocardiumMyocytes, CardiacRatsSwineDetergentsLidocaineCardiac tissue engineeringCell cultureElectrophysiologyExtracellular matrixScaffold

Identifiers

PMID25934457
PMCPMC4780840

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.