ArticlePloS one2015
Epstein-Barr virus genetic variation in lymphoblastoid cell lines derived from Kenyan pediatric population.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 15 papers.
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The trial behind it
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Who cites it
15 citing papers in PubMed.
- Distribution of Epstein-Barr Virus LMP1 Variants in Patients with Infectious Mononucleosis and Association with Selected Biochemical and Hematological Parameters.Pathogens (Basel, Switzerland) · 2023Article
- Unique synapomorphies and high diversity in South American Raji-related Epstein-Barr virus genomes.Memorias do Instituto Oswaldo Cruz · 2023Article
- Molecular Characterisation of Epstein-Barr Virus in Classical Hodgkin Lymphoma.International journal of molecular sciences · 2022Article
- Whole genome sequence analysis of equid gammaherpesvirus -2 field isolates reveals high levels of genomic diversity and recombination.BMC genomics · 2022Article
- CD21 (Complement Receptor 2) Is the Receptor for Epstein-Barr Virus Entry into T Cells.Journal of virology · 2020Article
- A Review of Cancer Genetics and Genomics Studies in Africa.Frontiers in oncology · 2020Review
- Multidimensional analysis of Gammaherpesvirus RNA expression reveals unexpected heterogeneity of gene expression.PLoS pathogens · 2019Article
- Metagenomic analysis of DNA viruses from posttransplant lymphoproliferative disorders.Cancer medicine · 2019Article
- Epstein-Barr Virus Type 2 Infects T Cells and Induces B Cell Lymphomagenesis in Humanized Mice.Journal of virology · 2018Article
- Evidence of EBV infection in lymphomas diagnosed in Lusaka, Zambia.The Pan African medical journal · 2018Article
- Heterogeneity of the Epstein-Barr Virus (EBV) Major Internal Repeat Reveals Evolutionary Mechanisms of EBV and a Functional Defect in the Prototype EBV Strain B95-8.Journal of virology · 2017Article
- Epstein-Barr Virus Type 2 Infects T Cells in Healthy Kenyan Children.The Journal of infectious diseases · 2017Article
- Natural Variation of Epstein-Barr Virus Genes, Proteins, and Primary MicroRNA.Journal of virology · 2017Article
- Review
- Correction: epstein-barr virus genetic variation in lymphoblastoid cell lines derived from Kenyan pediatric population.PloS one · 2015Article
Corrections and comments
- Erratum issued
Authors and funding
8 authors.
Funding
Abstract
Epstein-Barr virus (EBV) is associated with Burkitt's lymphoma (BL), and in regions of sub-Saharan Africa where endemic BL is common, both the EBV Type 1 (EBV-1) and EBV Type 2 strains (EBV-2) are found. Little is known about genetic variation of EBV strains in areas of sub-Saharan Africa. In the present study, spontaneous lymphoblastoid cell lines (LCLs) were generated from samples obtained from Kenya. Polymerase chain reaction (PCR) amplification of the EBV genome was done using multiple primers and sequenced by next-generation sequencing (NGS). Phylogenetic analyses against the published EBV-1 and EBV-2 strains indicated that one sample, LCL10 was closely related to EBV-2, while the remaining 3 LCL samples were more closely related to EBV-1. Moreover, single nucleotide polymorphism (SNP) analyses showed clustering of LCL variants. We further show by analysis of EBNA-1, BLLF1, BPLF1, and BRRF2 that latent genes are less conserved than lytic genes in these LCLs from a single geographic region. In this study we have shown that NGS is highly useful for deciphering detailed inter and intra-variations in EBV genomes and that within a geographic region different EBV genetic variations can co-exist, the implications of which warrant further investigation. The findings will enhance our understanding of potential pathogenic variants critical to the development and maintenance of EBV-associated malignancies.
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