ArticlePloS one2015
Digital Microfluidic Dynamic Culture of Mammalian Embryos on an Electrowetting on Dielectric (EWOD) Chip.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Microfluidic chips in female reproduction: a systematic review of status, advances, and challenges.Theranostics · 2024Pooled it
- Microfluidic Fibroblast Cell Culture Chip for Embryo Co-Culture: Analysis of Preimplantation Embryo Viability and Development Potential.International journal of molecular sciences · 2026Article
- Mechanical cues regulateMaterials today. Bio · 2026Article
- Embryo metabolite analysis and implantation potential prediction using chemiluminescent microfluidic chips with dielectric wetting valves.Nature communications · 2026Article
- Microfluidics for in vitro fertilization: from science to clinical validation.Human reproduction update · 2026Review
- Review
- Revolutionizing the female reproductive system research using microfluidic chip platform.Journal of nanobiotechnology · 2023Review
- Development of a Microfluidic Chip Powered by EWOD for In Vitro Manipulation of Bovine Embryos.Biosensors · 2023Article
- Opportunities involving microfluidics and 3D culture systems to theAnimal reproduction · 2023Review
- Contactless Micro-Droplet Manipulation of Liquid Released from a Parallel Plate to an Open Region in Electrowetting-on-Dielectric Platform.Micromachines · 2022Article
- Surface functionalization of poly(dimethylsiloxane) substrates facilitates culture of pre-implantation mouse embryos by blocking non-selective adsorption.Journal of the Royal Society, Interface · 2022Article
- Identification of Microorganisms Using an EWOD System.Micromachines · 2022Article
- Mechanobiology of the female reproductive system.Reproductive medicine and biology · 2021Review
- A Review on Microfluidics: An Aid to Assisted Reproductive Technology.Molecules (Basel, Switzerland) · 2021Review
- Toward embryo cryopreservation-on-a-chip: A standalone microfluidic platform for gradual loading of cryoprotectants to minimize cryoinjuries.Biomicrofluidics · 2021Article
- Biophysical optimization of preimplantation embryo culture: what mechanics can offer ART.Molecular human reproduction · 2021Review
- Recent advances in critical nodes of embryo engineering technology.Theranostics · 2021Review
- Recent Advances on the Model, Measurement Technique, and Application of Single Cell Mechanics.International journal of molecular sciences · 2020Review
- Using a Dielectrophoretic Microfluidic Biochip Enhanced Fertilization of Mouse Embryo in Vitro.Micromachines · 2020Article
- Extraction of Cell-free Dna from An Embryo-culture Medium Using Micro-scale Bio-reagents on Ewod.Scientific reports · 2020Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Current human fertilization in vitro (IVF) bypasses the female oviduct and manually inseminates, fertilizes and cultivates embryos in a static microdrop containing appropriate chemical compounds. A microfluidic microchannel system for IVF is considered to provide an improved in-vivo-mimicking environment to enhance the development in a culture system for an embryo before implantation. We demonstrate a novel digitalized microfluidic device powered with electrowetting on a dielectric (EWOD) to culture an embryo in vitro in a single droplet in a microfluidic environment to mimic the environment in vivo for development of the embryo and to culture the embryos with good development and live births. Our results show that the dynamic culture powered with EWOD can manipulate a single droplet containing one mouse embryo and culture to the blastocyst stage. The rate of embryo cleavage to a hatching blastocyst with a dynamic culture is significantly greater than that with a traditional static culture (p<0.05). The EWOD chip enhances the culture of mouse embryos in a dynamic environment. To test the reproductive outcome of the embryos collected from an EWOD chip as a culture system, we transferred embryos to pseudo-pregnant female mice and produced live births. These results demonstrate that an EWOD-based microfluidic device is capable of culturing mammalian embryos in a microfluidic biological manner, presaging future clinical application.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.