Evidence map›Paper›PMID 25932286›Full record

ArticleProceedings of SPIE--the International Society for Optical Engineering2015

Three dimensional time-gated tracking of non-blinking quantum dots in live cells.

Matthew S DeVore, Dominik G Stich, Aaron M Keller, Yagnaseni Ghosh, Peter M Goodwin, Mary E Phipps, Michael H Stewart, Cédric Cleyrat, Bridget S Wilson, Diane S Lidke and 2 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Proceedings of SPIE--the International Society for Optical Engineering, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
2.5field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 8 citations in OpenAlex.

  1. Recent Advances in Single-Molecule Tracking and Imaging Techniques.Annual review of analytical chemistry (Palo Alto, Calif.) · 2023
    Review
  2. Article
  3. Single-Molecule Tracking and Its Application in Biomolecular Binding Detection.IEEE journal of selected topics in quantum electronics : a publication of the IEEE Lasers and Electro-optics Society
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 4 institutions in 1 country.

Matthew S DeVoreCenter for Integrated Nanotechnologies, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, USA.
Dominik G StichCenter for Integrated Nanotechnologies, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, USA.
Aaron M KellerCenter for Integrated Nanotechnologies, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, USA.
Yagnaseni GhoshCenter for Integrated Nanotechnologies, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, USA.
Peter M GoodwinCenter for Integrated Nanotechnologies, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, USA.
Mary E PhippsCenter for Integrated Nanotechnologies, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, USA.
Michael H StewartOptical Sciences Division, Code 5600, U.S. Naval Research Laboratory, Washington D.C. 20375, USA.
Cédric CleyratDepartment of Pathology and Cancer Research and Treatment Center, University of New Mexico, Albuquerque, NM 87131, USA.
Bridget S WilsonDepartment of Pathology and Cancer Research and Treatment Center, University of New Mexico, Albuquerque, NM 87131, USA.
Diane S LidkeDepartment of Pathology and Cancer Research and Treatment Center, University of New Mexico, Albuquerque, NM 87131, USA.
Jennifer A HollingsworthCenter for Integrated Nanotechnologies, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, USA.
James H WernerCenter for Integrated Nanotechnologies, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, USA.
Los Alamos National Laboratory · USCenter for Integrated Nanotechnologies · USNew Mexico Cancer Center · USUniversity of New Mexico · US

Funding

Mapping for Specialized Domains for FCeRI Signaling & InternalizationR01AI051575 · NIAID · UNIVERSITY OF NEW MEXICO HEALTH SCIS CTR · PI WILSON, BRIDGET S · 2003 to 2013
$3.3M
Three-Dimensional Molecular Tracking of IgE-Fc{epsilon}RI in Live CellsR01AI097154 · NIAID · LOS ALAMOS NAT SECTY-LOS ALAMOS NAT LAB · PI WERNER, JAMES H · 2012 to 2015
$1.6M
Single Molecule Imaging to Quantify FcεRI Signaling DynamicsR01GM100114 · NIGMS · UNIVERSITY OF NEW MEXICO HEALTH SCIS CTR · PI LIDKE, DIANE · 2012 to 2016
$1.4M
Functional Analysis of the Sulfotransferase, SULT2B1F32GM065794 · NIGMS · INSTITUTE FOR CANCER RESEARCH · PI GEESE, WILLIAM J · 2002 to 2002
$31k
NIAID NIH HHS R01 AI051575NIAID NIH HHS R01 AI097154NIGMS NIH HHS F32 GM065794NIGMS NIH HHS R01 GM100114
6 · The paper itself

Abstract

Single particle tracking has provided a wealth of information about biophysical processes such as motor protein transport and diffusion in cell membranes. However, motion out of the plane of the microscope or blinking of the fluorescent probe used as a label generally limits observation times to several seconds. Here, we overcome these limitations by using novel non-blinking quantum dots as probes and employing a custom 3D tracking microscope to actively follow motion in three dimensions (3D) in live cells. Signal-to-noise is improved in the cellular milieu through the use of pulsed excitation and time-gated detection.

Indexed as

microscopyquantum dotSingle particle trackingtime-gating

Identifiers

PMID25932286
PMCPMC4412170
OpenAlexW2086177734

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.