Evidence map›Paper›PMID 25931306›Full record

ArticleJournal of controlled release : official journal of the Controlled Release Society2015

Molecular weight and concentration of heparin in hyaluronic acid-based matrices modulates growth factor retention kinetics and stem cell fate.

Amit K Jha, Anurag Mathur, Felicia L Svedlund, Jianqin Ye, Yerem Yeghiazarians, Kevin E Healy

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of controlled release : official journal of the Controlled Release Society, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.

0numbers the graph read from it
0cells of the map it votes in
30citing papers in PubMed
4.1field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

30 citing papers in PubMed, 82 citations in OpenAlex.

  1. Review
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  5. Article
  6. Article
  7. Unlocking the Potential of Stem Cell Microenvironments In Vitro.Bioengineering (Basel, Switzerland) · 2024
    Review
  8. Review
  9. Review
  10. Review
  11. Review
  12. Article
  13. Review
  14. Article
  15. Review
  16. Review
  17. Article
  18. Immobilization of Growth Factors for Cell Therapy Manufacturing.Frontiers in bioengineering and biotechnology · 2020
    Review
  19. Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Amit K JhaDepartment of Bioengineering, University of California, Berkeley, CA 94720, USA; Department of Material Science and Engineering, University of California, Berkeley, CA 94720, USA.
Anurag MathurDepartment of Bioengineering, University of California, Berkeley, CA 94720, USA; Department of Material Science and Engineering, University of California, Berkeley, CA 94720, USA.
Felicia L SvedlundDepartment of Material Science and Engineering, University of California, Berkeley, CA 94720, USA.
Jianqin YeDepartment of Medicine, University of California, San Francisco, CA 94143, USA.
Yerem YeghiazariansDepartment of Medicine, University of California, San Francisco, CA 94143, USA; Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California, San Francisco, CA 94143, USA; Cardiovascular Research Institute, University of California, San Francisco, CA 94143, USA.
Kevin E HealyDepartment of Bioengineering, University of California, Berkeley, CA 94720, USA; Department of Material Science and Engineering, University of California, Berkeley, CA 94720, USA. Electronic address: kehealy@berkeley.edu.
University of California, Berkeley · USUniversity of California, San Francisco · US

Funding

Enabling Technologies for hES Cell Self-RenewalR01HL096525 · NHLBI · UNIVERSITY OF CALIFORNIA BERKELEY · PI HEALY, KEVIN EDWARD · 2009 to 2013
$1.8M
Matrix assisted cell transplantation of brown fatR01DK101293 · NIDDK · UNIVERSITY OF CALIFORNIA BERKELEY · PI HEALY, KEVIN EDWARD, STAHL, ANDREAS · 2014 to 2017
$1.7M
NHLBI NIH HHS R01 HL096525NHLBI NIH HHS R01HL096525NIDDK NIH HHS R01 DK101293
6 · The paper itself

Abstract

Growth factors are critical for regulating and inducing various stem cell functions. To study the effects of growth factor delivery kinetics and presentation on stem cell fate, we developed a series of heparin-containing hyaluronic acid (HyA)-based hydrogels with various degrees of growth factor affinity and retention. To characterize this system, we investigated the effect of heparin molecular weight, fractionation, and relative concentration on the loading efficiency and retention kinetics of TGFβ1 as a model growth factor. At equal concentrations, high MW heparin both loaded and retained the greatest amount of TGFβ1, and had the slowest release kinetics, primarily due to the higher affinity with TGFβ1 compared to low MW or unfractionated heparin. Subsequently, we tested the effect of TGFβ1, presented from various heparin-containing matrices, to differentiate a versatile population of Sca-1(+)/CD45(-) cardiac progenitor cells (CPCs) into endothelial cells and form vascular-like networks in vitro. High MW heparin HyA hydrogels stimulated more robust differentiation of CPCs into endothelial cells, which formed vascular-like networks within the hydrogel. This observation was attributed to the ability of high MW heparin HyA hydrogels to sequester endogenously synthesized angiogenic factors within the matrix. These results demonstrate the importance of molecular weight, fractionation, and concentration of heparin on presentation of heparin-binding growth factors and their effect on stem cell differentiation and lineage specification.

Indexed as

AnimalsCell DifferentiationCells, CulturedHeparinHyaluronic AcidHydrogelsKineticsMiceMolecular WeightStem CellsTransforming Growth Factor beta1HeparinHyaluronic AcidHydrogelsTransforming Growth Factor beta1Cardiac progenitor cellGrowth factor sequestrationHMWHHyaluronic acid hydrogelLMWHNeovascularizationTGFβ1UMWH

Identifiers

PMID25931306
PMCPMC4465536
OpenAlexW2044048391

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.