ArticleStem cell reviews and reports2015
Increased In Vitro Osteopotential in SHED Associated with Higher IGF2 Expression When Compared with hASCs.
Article in Stem cell reviews and reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 30 citations in OpenAlex.
- SHEDs and BMSCs exhibit distinct lineage preferences in HUVECs dynamic spheroid co-cultures: vascular versus osteogenic commitment.Bioengineering & translational medicine · 2026Article
- PLAGL1-IGF2 axis regulates osteogenesis of postnatal condyle development.International journal of oral science · 2025Article
- Effect of DNA methylation on the osteogenic differentiation of mesenchymal stem cells: concise review.Frontiers in genetics · 2024Review
- Repopulation of a 3D simulated periapical lesion cavity with dental pulp stem cell spheroids with triggered osteoblastic differentiation.Brazilian dental journal · 2024Article
- GJB2 c.35del variant up-regulates GJA1 gene expression and affects differentiation of human stem cells.Genetics and molecular biology · 2024Article
- Long noncoding RNA (lncRNA)Genes & diseases · 2023Review
- Review
- Dental pulp stem cells as a promising model to study imprinting diseases.International journal of oral science · 2022Article
- Adult mesenchymal stem cells and their possibilities for Dentistry: what to expect?Dental press journal of orthodontics · 2020Article
- Adipogenesis, Osteogenesis, and Chondrogenesis of Human Mesenchymal Stem/Stromal Cells: A Comparative Transcriptome Approach.Frontiers in cell and developmental biology · 2020Review
- Potential Research Tool of Stem Cells from Human Exfoliated Deciduous Teeth: Lentiviral Bmi-1 Immortalization with EGFP Marker.Stem cells international · 2019Article
- Is There a Noninvasive Source of MSCs Isolated with GMP Methods with Better Osteogenic Potential?Stem cells international · 2019Article
- The Roles of Insulin-Like Growth Factors in Mesenchymal Stem Cell Niche.Stem cells international · 2017Review
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mesenchymal stem cell (MSC) osteogenic differentiation potential varies according to factors such as tissue source and cell population heterogeneity. Pre-selection of cell subpopulations harboring higher osteopotential is a promising strategy to achieve a thorough translation of MSC-based therapies to the clinic. Here, we searched for novel molecular markers predictive of osteopotential by comparing MSC populations from two sources harboring different osteogenic potentials. We show that MSCs from human deciduous teeth (SHED) have an intrinsically higher osteogenic potential when compared with MSCs from human adipose tissue (hASCs) under the same in vitro controlled induction system. Transcriptome profiling revealed IGF2 to be one of the top upregulated transcripts before and during early in vitro osteogenic differentiation. Further, exogenous IGF2 supplementation enhanced alkaline phosphatase activity and matrix mineralization, and inhibition of IGF2 lessened these parameters in SHED and hASCs, validating IGF2 as an osteogenic factor in these MSCs. Further, we found IGF2 to be biallelically expressed in SHED, but not in hASCs. We observed a 4 % methylation increase in the imprinting control region within the IGF2-H19 locus in SHED, and this is mainly due to 2 specific CpG sites. Thus, we suggest that IGF2 upregulation in SHED is due to loss of imprinting. This study unravels osteogenic properties in SHED, implying IGF2 as a potential biomarker of MSCs with higher osteopotential, and unveils IGF2 loss-of-imprinting in SHED.
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