ArticleThe Journal of allergy and clinical immunology2015
Analysis of glutathione S-transferase allergen cross-reactivity in a North American population: Relevance for molecular diagnosis.
Article in The Journal of allergy and clinical immunology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 59 citations in OpenAlex.
- Exploring and Mitigating Insecticide Resistance in Urban Pests with New Synergists and Insecticides.Insects · 2026Review
- Cockroach sensitization and its hidden links to mite and food allergens.Scientific reports · 2026Article
- Cockroach allergy: Understanding complex immune responses to develop novel therapies.Molecular immunology · 2023Article
- Structure and IgE Cross-Reactivity among Cashew, Pistachio, Walnut, and Peanut Vicilin-Buried Peptides.Journal of agricultural and food chemistry · 2023Article
- The Molecular Allergen Recognition Profile in China as Basis for Allergen-Specific Immunotherapy.Frontiers in immunology · 2021Review
- Allergological Importance of Invertebrate Glutathione Transferases in Tropical Environments.Frontiers in allergy · 2021Review
- The allergenic activity and clinical impact of individual IgE-antibody binding molecules from indoor allergen sources.The World Allergy Organization journal · 2020Review
- Cross-reactivity in allergy: A double-edged sword.Allergy · 2020Article
- Structural Aspects of the Allergen-Antibody Interaction.Frontiers in immunology · 2020Review
- Cockroach allergen component analysis of children with or without asthma and rhinitis in an inner-city birth cohort.The Journal of allergy and clinical immunology · 2019Observational
- Allergen content in German cockroach extracts and sensitization profiles to a new expanded set of cockroach allergens determine in vitro extract potency for IgE reactivity.The Journal of allergy and clinical immunology · 2019Article
- Structural Analysis of Recent Allergen-Antibody Complexes and Future Directions.Current allergy and asthma reports · 2019Review
- Keeping Allergen Names Clear and Defined.Frontiers in immunology · 2019Review
- Assessment of Fasciola hepatica glutathione S-transferase as an antigen for serodiagnosis of human chronic fascioliasis.Acta tropica · 2018Article
- Influence of Parasitic Worm Infections on Allergy Diagnosis in Sub-Saharan Africa.Current allergy and asthma reports · 2017Review
- New Insights into Cockroach Allergens.Current allergy and asthma reports · 2017Review
- Allergens of Blomia tropicalis: An Overview of Recombinant Molecules.International archives of allergy and immunology · 2017Review
- Contributions and Future Directions for Structural Biology in the Study of Allergens.International archives of allergy and immunology · 2017Review
- Human Helminths and Allergic Disease: The Hygiene Hypothesis and Beyond.The American journal of tropical medicine and hygiene · 2016Review
- Epitope Mapping of Rhi o 1 and Generation of a Hypoallergenic Variant: A CANDIDATE MOLECULE FOR FUNGAL ALLERGY VACCINES.The Journal of biological chemistry · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 4 institutions in 3 countries.
Funding
Abstract
backgroundIt is not clear whether cross-reactivity or cosensitization to glutathione S-transferases (GSTs) occurs in tropical and subtropical environments. In the United States, Bla g 5 is the most important GST allergen and lack of coexposure to GSTs from certain species allows a better assessment of cross-reactivity.
objectivesTo examine the molecular structure of GST allergens from cockroach (Bla g 5), dust mites (Der p 8 and Blo t 8), and helminth (Asc s 13) for potential cross-reactive sites, and to assess the IgE cross-reactivity of sensitized patients from a temperate climate for these allergens for molecular diagnostic purposes.
methodsFour crystal structures were determined. Sera from patients allergic to cockroach and mite were tested for IgE reactivity to these GSTs. A panel of 6 murine anti-Bla g 5 mAb was assessed for cross-reactivity with the other 3 GSTs using antibody binding assays.
resultsComparisons of the allergen structures, formed by 2-domain monomers that dimerize, revealed few contiguous regions of similar exposed residues, rendering cross-reactivity unlikely. Accordingly, anti-Bla g 5 or anti-Der p 8 IgE from North American patients did not recognize Der p 8 or Bla g 5, respectively, and neither showed binding to Blo t 8 or Asc s 13. A weaker binding of anti-Bla g 5 IgE to Der p 8 versus Bla g 5 (∼ 100-fold) was observed by inhibition assays, similar to a weak recognition of Der p 8 by anti-Bla g 5 mAb. Patients from tropical Colombia had IgE to all 4 GSTs.
conclusionsThe lack of significant IgE cross-reactivity among the 4 GSTs is in agreement with the low shared amino acid identity at the molecular surface. Each GST is needed for accurate molecular diagnosis in different geographic areas.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.