ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2015
Characterization of Soybean mosaic virus resistance derived from inverted repeat-SMV-HC-Pro genes in multiple soybean cultivars.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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29 citing papers in PubMed, 60 citations in OpenAlex.
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- Overexpression of the Wild Soybean Expansin GenePlants (Basel, Switzerland) · 2025Article
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- Transgenic expression of artificial microRNA targeting soybean mosaic virus P1 gene confers virus resistance in plant.Transgenic research · 2024Article
- Comprehensive analysis of soybean cultivars' response to SMV infection: genotypic association, molecular characterization, and defense gene expressions.Journal, genetic engineering & biotechnology · 2023Article
- Major viral diseases in grain legumes: designing disease resistant legumes from plant breeding and OMICS integration.Frontiers in plant science · 2023Review
- Development of Comprehensive Serological Techniques for Sensitive, Quantitative and Rapid Detection ofInternational journal of molecular sciences · 2022Article
- Decades of Genetic Research onViruses · 2022Review
- RNA Interference: Promising Approach to Combat Plant Viruses.International journal of molecular sciences · 2022Review
- Pathogenicity and genome-wide sequence analysis reveals relationships between soybean mosaic virus strains.Archives of virology · 2022Article
- Optimizing RNAi-Target byFrontiers in plant science · 2021Article
- A major gene for tolerance to cold-induced seed coat discoloration relieves viral seed mottling in soybean.Breeding science · 2020Article
- Synthesis of Full-Length cDNA Infectious Clones ofViruses · 2020Article
- Transgenic plant generated by RNAi-mediated knocking down of soybean Vma12 and soybean mosaic virus resistance evaluation.AMB Express · 2020Article
- Soybean RNA interference lines silenced for eIF4E show broad potyvirus resistance.Molecular plant pathology · 2020Article
- Soybean Resistance to Soybean Mosaic Virus.Plants (Basel, Switzerland) · 2020Review
- Engineering plant virus resistance: from RNA silencing to genome editing strategies.Plant biotechnology journal · 2020Review
- High-throughput sequencing reveals the molecular mechanisms determining the stay-green characteristic in soybeans.Journal of biosciences · 2020Article
- Review
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
key messageSoybean mosaic virus resistance was significantly improved in multiple soybean cultivars through genetic transformation induced by inverted repeat-SMV- HC - Pro genes based on RNAi and post-transcriptional gene silencing. Here, we demonstrate Soybean mosaic virus (SMV) resistance in transgenic soybean plants. Transformation of five soybean genotypes with a construct containing inverted repeat-SMV-HC-Pro genes-induced high-level SMV resistance. Through leaf-painting assays, polymerase chain reaction (PCR) verification and LibertyLink(®) strip detection, 105 T0 and 1059 T1 plants were confirmed as transgene-positive. Southern blotting confirmed insertion of the T-DNA into the genomic DNA and revealed a low-copy integration pattern. Most T0 plants were fertile and transmitted the exogenous genes to their progenies (ratios of 3:1 or 15:1). In the T1 generation, virus resistance was evaluated visually after inoculation with SMV (strain SC3) and 441 plants were highly resistant (HR). SMV disease rating was classified on a scale with 0 = symptomless and 4 = mosaic symptoms with severe leaf curl. In the positive T1 plants, the disease rating on average was 1.42 (range 0.45-2.14) versus 3.2 (range 2-4) for the nontransformed plants. With the T2 generation, 75 transgene-positive plants were inoculated with SC3, and 57 HR plants were identified. Virus-induced seed coat mottling was eliminated in the resistant lines. Analysis of SMV levels in the plants was performed using quantitative real-time PCR and double-antibody sandwich enzyme-linked immunosorbent assays; the results revealed no virus or a gradual reduction over time in the viral content, thereby supporting the visual examination results. This is the first report demonstrating pathogen-derived resistance to SMV induced by inverted repeat-SMV-HC-Pro genes in multiple soybean cultivars. Our findings contribute positively to the study of transgenic SMV-resistance using RNA interference.
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