ArticleACS nano2015
Using surface enhanced Raman scattering to analyze the interactions of protein receptors with bacterial quorum sensing modulators.
Article in ACS nano, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- In silico evaluation of FDA-approved antivirals and corticosteroids against SARS-CoV-2.Scientific reports · 2026Article
- SERS-Based Immunoassay for α-Fetoprotein Biomarker Detection Using an Au-Ag Nanostars Platform.Biosensors · 2025Article
- Extracellular aminopeptidase regulates exopolysaccharide production of Pseudomonas aeruginosa via quorum sensing.The ISME journal · 2025Article
- Black Silicon: Breaking through the Everlasting Cost vs. Effectivity Trade-Off for SERS Substrates.Materials (Basel, Switzerland) · 2023Article
- Biophysical Approaches for the Characterization of Protein-Metabolite Interactions.Methods in molecular biology (Clifton, N.J.) · 2023Review
- Anti-quorum sensing activity ofBioinformation · 2023Article
- The Association between Biofilm Formation and Antimicrobial Resistance with Possible Ingenious Bio-Remedial Approaches.Antibiotics (Basel, Switzerland) · 2022Review
- Use of Surface-Enhanced Raman Scattering (SERS) Probes to Detect Fatty Acid Receptor Activity in a Microfluidic Device.Sensors (Basel, Switzerland) · 2019Article
- Small Molecule Anti-biofilm Agents Developed on the Basis of Mechanistic Understanding of Biofilm Formation.Frontiers in chemistry · 2019Review
- Terrein is an inhibitor of quorum sensing and c-di-GMP in Pseudomonas aeruginosa: a connection between quorum sensing and c-di-GMP.Scientific reports · 2018Article
- Surface-Enhanced Raman Scattering Spectroscopy for Label-Free Analysis ofFrontiers in cellular and infection microbiology · 2018Review
- Spatially multiplexed dark-field microspectrophotometry for nanoplasmonics.Scientific reports · 2016Article
- Ultrasensitive surface-enhanced Raman scattering detection in common fluids.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- A Comparative Analysis of Synthetic Quorum Sensing Modulators in Pseudomonas aeruginosa: New Insights into Mechanism, Active Efflux Susceptibility, Phenotypic Response, and Next-Generation Ligand Design.Journal of the American Chemical Society · 2015Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Many members of the LuxR family of quorum sensing (QS) transcriptional activators, including LasR of Pseudomonas aeruginosa, are believed to require appropriate acyl-homoserine lactone (acyl-HSL) ligands to fold into an active conformation. The failure to purify ligand-free LuxR homologues in nonaggregated form at the high concentrations required for their structural characterization has limited the understanding of the mechanisms by which QS receptors are activated. Surface-enhanced Raman scattering (SERS) is a vibrational spectroscopy technique that can be applied to study proteins at extremely low concentrations in their active state. The high sensitivity of SERS has allowed us to detect molecular interactions between the ligand-binding domain of LasR (LasRLBD) as a soluble apoprotein and modulators of P. aeruginosa QS. We found that QS activators and inhibitors produce differential SERS fingerprints in LasRLBD, and in combination with molecular docking analysis provide insight into the relevant interaction mechanism. This study reveals signal-specific structural changes in LasR upon ligand binding, thereby confirming the applicability of SERS to analyze ligand-induced conformational changes in proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.