ArticleFrontiers in microbiology2015
Microbial growth and physiology: a call for better craftsmanship.
Article in Frontiers in microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed, 123 citations in OpenAlex.
- Confronting Physical, Chemical and Temporal Biases in Experimental Algal Biology.International journal of molecular sciences · 2026Review
- Enhancing the E. coli Cell Density Expressing the Recombinant Cellulolytic Chimeric Enzyme from Acetivibrio thermocellus by Statistical Optimization of the Medium.Current microbiology · 2026Article
- Advancing mold identification in the routine laboratory, performance of smartphone-based imaging and a newly developed convolutional neural network.Microbiology spectrum · 2026Article
- Time Series Data Mining for Linking the Shape of Bacterial Growth Curves to Biological Functions.Computational and structural biotechnology journal · 2026Article
- Development of a mathematical model for microbial consumption of glucose and fructose during cocoa (Microbiology spectrum · 2025Article
- Short- and Long-Term Evolution of Microbial Cultures: A Thermodynamic Perspective.International journal of molecular sciences · 2025Article
- Trace elements increase replicability of microbial growth.Open biology · 2025Article
- Gradient boosted regression as a tool to reveal key drivers of temporal dynamics in a synthetic yeast community.FEMS microbiology ecology · 2024Article
- Insights into the rapid metabolism of Geobacillus sp. LC300: unraveling metabolic requirements and optimal growth conditions.Extremophiles : life under extreme conditions · 2023Article
- Kinetic and stoichiometric parameters in the fed-batch bioreactor production of poly(3-hydroxybutyrate) by Bacillus megaterium using different carbon sources.Bioprocess and biosystems engineering · 2023Article
- Impact of malt concentration in solid substrate on mycelial growth and network connectivity in Ganoderma species.Scientific reports · 2023Article
- Influence of redox potential on the accumulation of poly(3-hydroxybutyrate) by Bacillus megaterium.Bioprocess and biosystems engineering · 2023Article
- Review
- Statistical optimization of a podoviral anti-MRSA phage CCASU-L10 generated from an under sampled repository: Chicken rinse.Frontiers in cellular and infection microbiology · 2023Article
- Sensor histidine kinasesFrontiers in cellular and infection microbiology · 2023Article
- Article
- Nutrient-Limited Operational Strategies for the Microbial Production of Biochemicals.Microorganisms · 2022Review
- Article
- Development of mixed starter culture for the fermentation of Ethiopian honey wine, Tej.Scientific reports · 2022Article
- Glaciers as microbial habitats: current knowledge and implication.Journal of microbiology (Seoul, Korea) · 2022Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Virtually every microbiological experiment starts with the cultivation of microbes. Consequently, as originally pointed out by Monod (1949), handling microbial cultures is a fundamental methodology of microbiology and mastering different cultivation techniques should be part of every microbiologist's craftsmanship. This is particularly important for research in microbial physiology, as the composition and behavior of microbes is strongly dependent on their growth environment. It has been pointed out repeatedly by eminent microbiologists that we should give more attention to the media and culturing conditions. However, this is obviously not adhered to with sufficient rigor as mistakes in basic cultivation principles are frequently found in the published research literature. The most frequent mistakes are the use of inappropriate growth media and little or no control of the specific growth rate, and some examples will be discussed here in detail. Therefore, this is a call for better microbiological craftsmanship when cultivating microbial cultures for physiological experiments. This call is not only addressed to researchers but it is probably even more important for the teaching of our discipline.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.