ArticleMolecular & cellular proteomics : MCP2015
Understanding the Mechanism of Thermotolerance Distinct From Heat Shock Response Through Proteomic Analysis of Industrial Strains of Saccharomyces cerevisiae.
Article in Molecular & cellular proteomics : MCP, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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21 citing papers in PubMed, 72 citations in OpenAlex.
- Identification of key transcription factors, including DAL80 and CRZ1, involved in heat and ethanol tolerance in Saccharomyces cerevisiae.Biotechnology for biofuels and bioproducts · 2025Article
- Comparative Genomics and Characterisation of the Role ofJournal of fungi (Basel, Switzerland) · 2025Article
- Thermotolerant yeasts promoting climate-resilient bioproduction.FEMS yeast research · 2025Review
- Elucidation and engineering mitochondrial respiratory-related genes for improving bioethanol production at high temperature inEngineering microbiology · 2024Article
- Response mechanisms of Saccharomyces cerevisiae to the stress factors present in lignocellulose hydrolysate and strategies for constructing robust strains.Biotechnology for biofuels and bioproducts · 2022Review
- Identification of a novel gene required for competitive growth at high temperature in the thermotolerant yeastMicrobiology (Reading, England) · 2022Article
- Mining novelFrontiers in microbiology · 2022Article
- Proteomic perspectives on thermotolerant microbes: an updated review.Molecular biology reports · 2022Review
- Proteomics of Two Thermotolerant Isolates ofJournal of fungi (Basel, Switzerland) · 2021Article
- Stress modulation as a means to improve yeasts for lignocellulose bioconversion.Applied microbiology and biotechnology · 2021Review
- A Hierarchical Transcriptional Regulatory Network Required for Long-Term Thermal Stress Tolerance in an IndustrialFrontiers in bioengineering and biotechnology · 2021Article
- Differential proteomic analysis by SWATH-MS unravels the most dominant mechanisms underlying yeast adaptation to non-optimal temperatures under anaerobic conditions.Scientific reports · 2020Article
- Article
- Stress-tolerant non-conventional microbes enable next-generation chemical biosynthesis.Nature chemical biology · 2020Review
- Reconstruction and analysis of a Kluyveromyces marxianus genome-scale metabolic model.BMC bioinformatics · 2019Article
- CRISPR/Cas-based screening of a gene activation library in Saccharomyces cerevisiae identifies a crucial role of OLE1 in thermotolerance.Microbial biotechnology · 2019Article
- Proteomic profiling and integrated analysis with transcriptomic data bring new insights in the stress responses ofBiotechnology for biofuels · 2019Article
- Nitric oxide increases biofilm formation inBiotechnology for biofuels · 2019Article
- QTL analysis reveals genomic variants linked to high-temperature fermentation performance in the industrial yeast.Biotechnology for biofuels · 2019Article
- The transcription factors Hsf1 and Msn2 of thermotolerantBiotechnology for biofuels · 2017Article
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Saccharomyces cerevisiae has been intensively studied in responses to different environmental stresses such as heat shock through global omic analysis. However, the S. cerevisiae industrial strains with superior thermotolerance have not been explored in any proteomic studies for elucidating the tolerance mechanism. Recently a new diploid strain was obtained through evolutionary engineering of a parental industrial strain, and it exhibited even higher resistance to prolonged thermal stress. Herein, we performed iTRAQ-based quantitative proteomic analysis on both the parental and evolved industrial strains to further understand the mechanism of thermotolerant adaptation. Out of ∼ 2600 quantifiable proteins from biological quadruplicates, 193 and 204 proteins were differentially regulated in the parental and evolved strains respectively during heat-stressed growth. The proteomic response of the industrial strains cultivated under prolonged thermal stress turned out to be substantially different from that of the laboratory strain exposed to sudden heat shock. Further analysis of transcription factors underlying the proteomic perturbation also indicated the distinct regulatory mechanism of thermotolerance. Finally, a cochaperone Mdj1 and a metabolic enzyme Adh1 were selected to investigate their roles in mediating heat-stressed growth and ethanol production of yeasts. Our proteomic characterization of the industrial strain led to comprehensive understanding of the molecular basis of thermotolerance, which would facilitate future improvement in the industrially important trait of S. cerevisiae by rational engineering.
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