Evidence map›Paper›PMID 25926513›Full record

ArticleThe American journal of clinical nutrition2015

Defining meal requirements for protein to optimize metabolic roles of amino acids.

Donald K Layman, Tracy G Anthony, Blake B Rasmussen, Sean H Adams, Christopher J Lynch, Grant D Brinkworth, Teresa A Davis

Abstract read
In one paragraph

Article in The American journal of clinical nutrition, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
57citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

57 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Effect of protein supplementation on hip bone mineral density, cortical thickness, and bone strength in older adult participants during a caloric restriction and aerobic exercise weight loss intervention: a randomized controlled trial.Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA · 2026
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  5. Improved Function With Enhanced Protein Intake per Meal: A Pilot Study of Weight Reduction in Frail, Obese Older Adults.The journals of gerontology. Series A, Biological sciences and medical sciences · 2016
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  14. Animal amino acid sensor - A review.Animal bioscience · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Donald K LaymanFrom the Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, Urbana, IL (DKL); the Department of Nutritional Sciences, Rutgers University, New Brunswick, NJ (TGA); the Department of Nutrition and Metabolism, Division of Rehabilitation Science, and Sealy Center on Aging, University of Texas Medical Branch, Galveston, TX (BBR); Arkansas Children's Nutrition Center and Department of Pediatrics, University of Arkansas for Medical Sciences, Little Rock, AR (SHA); the Department of Cellular and Molecular Physiology, the Pennsylvania State University College of Medicine, Hershey, PA (CJL); the Commonwealth Scientific and Industrial Research Organization-Food and Nutritional Sciences, Adelaide, Australia (GDB); and the USDA-Agricultural Research Service Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, TX (TAD).
Tracy G AnthonyFrom the Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, Urbana, IL (DKL); the Department of Nutritional Sciences, Rutgers University, New Brunswick, NJ (TGA); the Department of Nutrition and Metabolism, Division of Rehabilitation Science, and Sealy Center on Aging, University of Texas Medical Branch, Galveston, TX (BBR); Arkansas Children's Nutrition Center and Department of Pediatrics, University of Arkansas for Medical Sciences, Little Rock, AR (SHA); the Department of Cellular and Molecular Physiology, the Pennsylvania State University College of Medicine, Hershey, PA (CJL); the Commonwealth Scientific and Industrial Research Organization-Food and Nutritional Sciences, Adelaide, Australia (GDB); and the USDA-Agricultural Research Service Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, TX (TAD).
Blake B RasmussenFrom the Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, Urbana, IL (DKL); the Department of Nutritional Sciences, Rutgers University, New Brunswick, NJ (TGA); the Department of Nutrition and Metabolism, Division of Rehabilitation Science, and Sealy Center on Aging, University of Texas Medical Branch, Galveston, TX (BBR); Arkansas Children's Nutrition Center and Department of Pediatrics, University of Arkansas for Medical Sciences, Little Rock, AR (SHA); the Department of Cellular and Molecular Physiology, the Pennsylvania State University College of Medicine, Hershey, PA (CJL); the Commonwealth Scientific and Industrial Research Organization-Food and Nutritional Sciences, Adelaide, Australia (GDB); and the USDA-Agricultural Research Service Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, TX (TAD).
Sean H AdamsFrom the Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, Urbana, IL (DKL); the Department of Nutritional Sciences, Rutgers University, New Brunswick, NJ (TGA); the Department of Nutrition and Metabolism, Division of Rehabilitation Science, and Sealy Center on Aging, University of Texas Medical Branch, Galveston, TX (BBR); Arkansas Children's Nutrition Center and Department of Pediatrics, University of Arkansas for Medical Sciences, Little Rock, AR (SHA); the Department of Cellular and Molecular Physiology, the Pennsylvania State University College of Medicine, Hershey, PA (CJL); the Commonwealth Scientific and Industrial Research Organization-Food and Nutritional Sciences, Adelaide, Australia (GDB); and the USDA-Agricultural Research Service Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, TX (TAD).
Christopher J LynchFrom the Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, Urbana, IL (DKL); the Department of Nutritional Sciences, Rutgers University, New Brunswick, NJ (TGA); the Department of Nutrition and Metabolism, Division of Rehabilitation Science, and Sealy Center on Aging, University of Texas Medical Branch, Galveston, TX (BBR); Arkansas Children's Nutrition Center and Department of Pediatrics, University of Arkansas for Medical Sciences, Little Rock, AR (SHA); the Department of Cellular and Molecular Physiology, the Pennsylvania State University College of Medicine, Hershey, PA (CJL); the Commonwealth Scientific and Industrial Research Organization-Food and Nutritional Sciences, Adelaide, Australia (GDB); and the USDA-Agricultural Research Service Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, TX (TAD).
Grant D BrinkworthFrom the Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, Urbana, IL (DKL); the Department of Nutritional Sciences, Rutgers University, New Brunswick, NJ (TGA); the Department of Nutrition and Metabolism, Division of Rehabilitation Science, and Sealy Center on Aging, University of Texas Medical Branch, Galveston, TX (BBR); Arkansas Children's Nutrition Center and Department of Pediatrics, University of Arkansas for Medical Sciences, Little Rock, AR (SHA); the Department of Cellular and Molecular Physiology, the Pennsylvania State University College of Medicine, Hershey, PA (CJL); the Commonwealth Scientific and Industrial Research Organization-Food and Nutritional Sciences, Adelaide, Australia (GDB); and the USDA-Agricultural Research Service Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, TX (TAD).
Teresa A DavisFrom the Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, Urbana, IL (DKL); the Department of Nutritional Sciences, Rutgers University, New Brunswick, NJ (TGA); the Department of Nutrition and Metabolism, Division of Rehabilitation Science, and Sealy Center on Aging, University of Texas Medical Branch, Galveston, TX (BBR); Arkansas Children's Nutrition Center and Department of Pediatrics, University of Arkansas for Medical Sciences, Little Rock, AR (SHA); the Department of Cellular and Molecular Physiology, the Pennsylvania State University College of Medicine, Hershey, PA (CJL); the Commonwealth Scientific and Industrial Research Organization-Food and Nutritional Sciences, Adelaide, Australia (GDB); and the USDA-Agricultural Research Service Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, TX (TAD).

Funding

Regulation of Neonatal Muscle Protein SynthesisR01AR044474 · NIAMS · BAYLOR COLLEGE OF MEDICINE · PI DAVIS, TERESA A · 1996 to 2012
$4.2M
Leucine Supplementation to Promote Lean Growth in Early LifeR01HD072891 · NICHD · BAYLOR COLLEGE OF MEDICINE · PI DAVIS, TERESA A · 2012 to 2015
$1.4M
NIAMS NIH HHS R01 AR044474NICHD NIH HHS R01 HD072891
6 · The paper itself

Abstract

Dietary protein provides essential amino acids (EAAs) for the synthesis of new proteins plus an array of other metabolic functions; many of these functions are sensitive to postprandial plasma and intracellular amino acid concentrations. Recent research has focused on amino acids as metabolic signals that influence the rate of protein synthesis, inflammation responses, mitochondrial activity, and satiety, exerting their influence through signaling systems including mammalian/mechanistic target of rapamycin complex 1 (mTORC1), general control nonrepressed 2 (GCN2), glucagon-like peptide 1 (GLP-1), peptide YY (PYY), serotonin, and insulin. These signals represent meal-based responses to dietary protein. The best characterized of these signals is the leucine-induced activation of mTORC1, which leads to the stimulation of skeletal muscle protein synthesis after ingestion of a meal that contains protein. The response of this metabolic pathway to dietary protein (i.e., meal threshold) declines with advancing age or reduced physical activity. Current dietary recommendations for protein are focused on total daily intake of 0.8 g/kg body weight, but new research suggests daily needs for older adults of ≥1.0 g/kg and identifies anabolic and metabolic benefits to consuming at least 20-30 g protein at a given meal. Resistance exercise appears to increase the efficiency of EAA use for muscle anabolism and to lower the meal threshold for stimulation of protein synthesis. Applying this information to a typical 3-meal-a-day dietary plan results in protein intakes that are well within the guidelines of the Dietary Reference Intakes for acceptable macronutrient intakes. The meal threshold concept for dietary protein emphasizes a need for redistribution of dietary protein for optimum metabolic health.

Indexed as

leucinemTORmuscle protein synthesisnitrogen balancesatiety

Identifiers

PMID25926513
PMCPMC5278948

What OpenQuestion holds

Textmetadata
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.