ArticleJournal of clinical microbiology2015
Escherichia coli O-Genotyping PCR: a Comprehensive and Practical Platform for Molecular O Serogrouping.
Article in Journal of clinical microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 81 papers.
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Who cites it
81 citing papers in PubMed.
- Prevalence of antimicrobial-resistantOne health (Amsterdam, Netherlands) · 2026Article
- A Large Outbreak of Gastrointestinal Infections Associated withFood safety (Tokyo, Japan) · 2026Article
- FirstMicrobiology spectrum · 2026Article
- Molecular Characterization and Antibiotic Resistance of Avian PathogenicAnimals : an open access journal from MDPI · 2025Article
- Emerging hybrid shigatoxigenic and enteropathogenicClinical microbiology reviews · 2025Review
- Co-resistance and plasmid-mediated co-dissemination of florfenicol and azithromycin resistance in non-O157 Shiga toxin-producing Escherichia coli from cattle in Xinjiang, China.BMC microbiology · 2025Article
- Shiga toxin-producing Escherichia coli plasmid diversity reveals virulence potential and control opportunities in animal hosts.Scientific reports · 2025Article
- A foodborne outbreak caused by atypical enteropathogenicApplied and environmental microbiology · 2025Article
- Comprehensive serotyping of Mannheimia haemolytica by a PCR system using the diversity of capsule biosynthesis genes.Scientific reports · 2025Article
- Infectious endophthalmitis associated with umbilical infection in Japanese black calf: a case report.Frontiers in veterinary science · 2025Article
- Extraintestinal Escherichia coli from camel carcasses: Phylogroups, serotypes, and markers of virulence.PloS one · 2025Article
- Molecular and in silico analyses for detection of Shiga toxin-producing Escherichia coli (STEC) and highly pathogenic enterohemorrhagic Escherichia coli (EHEC) using genetic markers located on plasmid, O Island 57 and O Island 71.BMC veterinary research · 2024Article
- Occurrence, serotypes and virulence characteristics of Shiga toxin-producing and Enteropathogenic Escherichia coli isolates from dairy cattle in South Africa.World journal of microbiology & biotechnology · 2024Article
- Inflammation, the kynurenines, and mucosal injury during human experimental enterotoxigenic Escherichia coli infection.Medical microbiology and immunology · 2024Article
- Combined usage of serodiagnosis and O antigen typing to isolate Shiga toxin-producingMicrobiology spectrum · 2024Article
- Antimicrobial growth promoters approved in food-producing animals in South Africa induce shiga toxin-converting bacteriophages from Escherichia coli O157:H7.Gut pathogens · 2023Article
- Atypical diarrhoeagenicEpidemiology and infection · 2023Article
- Virulence of Shigatoxigenic and EnteropathogenicVeterinary sciences · 2023Article
- Co-Occurrence ofAntibiotics (Basel, Switzerland) · 2023Article
- Comparative Genetic Characterization of PathogenicAnimals : an open access journal from MDPI · 2023Article
21 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The O serogrouping of pathogenic Escherichia coli is a standard method for subtyping strains for epidemiological studies and enhancing phylogenetic studies. In particular, the identification of strains of the same O serogroup is essential in outbreak investigations and surveillance. In a previous study, we analyzed the O-antigen biosynthesis gene cluster in all known E. coli O serogroups (A. Iguchi et al., DNA Res, 22:101-107, 2015, http://dx.doi.org/10.1093/dnares/dsu043). Based on those results, we have arranged 162 PCR primer pairs for the identification or classification of O serogroups. Of these, 147 pairs were used to identify 147 individual O serogroups with unique O-antigen biosynthesis genes, and the other 15 pairs were used to identify 15 groups of strains (Gp1 to Gp15). Each of these groups consisted of strains with identical or very similar O-antigen biosynthesis genes, and the groups represented a total of 35 individual O serogroups. We then used the 162 primer pairs to create 20 multiplex PCR sets. Each set contained six to nine primer pairs that amplify products of markedly different sizes. This genetic methodology (E. coli O-genotyping PCR) allowed for comprehensive, rapid, and low-cost typing. Validation of the PCR system using O-serogroup references and wild strains showed that the correct O serogroups were specifically and accurately identified for 100% (182/182) and 90.8% (522/575) of references and wild strains, respectively. The PCR-based system reported here might be a promising tool for the subtyping of E. coli strains for epidemiological studies as well as for the surveillance of pathogenic E. coli during outbreaks.
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