Evidence map›Paper›PMID 25926482›Full record

ArticlePlant physiology2015

TRANSPARENT TESTA GLABRA1 and GLABRA1 Compete for Binding to GLABRA3 in Arabidopsis.

Martina Pesch, Ilka Schultheiß, Karsten Klopffleisch, Joachim F Uhrig, Manfred Koegl, Christoph S Clemen, Rüdiger Simon, Stefanie Weidtkamp-Peters, Martin Hülskamp

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.

0numbers the graph read from it
0cells of the map it votes in
40citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

40 citing papers in PubMed.

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  8. Transcription FactorInternational journal of molecular sciences · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Martina PeschBiocenter, Cologne University, Botanical Institute, 50674 Cologne, Germany (M.P., I.S., K.K., J.F.U., M.H.);Preclinical Target Development and Genomics and Proteomics Core Facilities, German Cancer Research Center, 69120 Heidelberg, Germany (M.K.);Center for Biochemistry, Institute of Biochemistry I, Medical Faculty, Cologne University, 50931 Cologne, Germany (C.S.C.); andHeinrich-Heine Universität Düsseldorf, 40225 Dusseldorf, Germany (R.S., S.W.-P.).
Ilka SchultheißBiocenter, Cologne University, Botanical Institute, 50674 Cologne, Germany (M.P., I.S., K.K., J.F.U., M.H.);Preclinical Target Development and Genomics and Proteomics Core Facilities, German Cancer Research Center, 69120 Heidelberg, Germany (M.K.);Center for Biochemistry, Institute of Biochemistry I, Medical Faculty, Cologne University, 50931 Cologne, Germany (C.S.C.); andHeinrich-Heine Universität Düsseldorf, 40225 Dusseldorf, Germany (R.S., S.W.-P.).
Karsten KlopffleischBiocenter, Cologne University, Botanical Institute, 50674 Cologne, Germany (M.P., I.S., K.K., J.F.U., M.H.);Preclinical Target Development and Genomics and Proteomics Core Facilities, German Cancer Research Center, 69120 Heidelberg, Germany (M.K.);Center for Biochemistry, Institute of Biochemistry I, Medical Faculty, Cologne University, 50931 Cologne, Germany (C.S.C.); andHeinrich-Heine Universität Düsseldorf, 40225 Dusseldorf, Germany (R.S., S.W.-P.).
Joachim F UhrigBiocenter, Cologne University, Botanical Institute, 50674 Cologne, Germany (M.P., I.S., K.K., J.F.U., M.H.);Preclinical Target Development and Genomics and Proteomics Core Facilities, German Cancer Research Center, 69120 Heidelberg, Germany (M.K.);Center for Biochemistry, Institute of Biochemistry I, Medical Faculty, Cologne University, 50931 Cologne, Germany (C.S.C.); andHeinrich-Heine Universität Düsseldorf, 40225 Dusseldorf, Germany (R.S., S.W.-P.).ORCID http://orcid.org/0000-0002-6592-4445
Manfred KoeglBiocenter, Cologne University, Botanical Institute, 50674 Cologne, Germany (M.P., I.S., K.K., J.F.U., M.H.);Preclinical Target Development and Genomics and Proteomics Core Facilities, German Cancer Research Center, 69120 Heidelberg, Germany (M.K.);Center for Biochemistry, Institute of Biochemistry I, Medical Faculty, Cologne University, 50931 Cologne, Germany (C.S.C.); andHeinrich-Heine Universität Düsseldorf, 40225 Dusseldorf, Germany (R.S., S.W.-P.).ORCID http://orcid.org/0000-0001-6633-9769
Christoph S ClemenBiocenter, Cologne University, Botanical Institute, 50674 Cologne, Germany (M.P., I.S., K.K., J.F.U., M.H.);Preclinical Target Development and Genomics and Proteomics Core Facilities, German Cancer Research Center, 69120 Heidelberg, Germany (M.K.);Center for Biochemistry, Institute of Biochemistry I, Medical Faculty, Cologne University, 50931 Cologne, Germany (C.S.C.); andHeinrich-Heine Universität Düsseldorf, 40225 Dusseldorf, Germany (R.S., S.W.-P.).
Rüdiger SimonBiocenter, Cologne University, Botanical Institute, 50674 Cologne, Germany (M.P., I.S., K.K., J.F.U., M.H.);Preclinical Target Development and Genomics and Proteomics Core Facilities, German Cancer Research Center, 69120 Heidelberg, Germany (M.K.);Center for Biochemistry, Institute of Biochemistry I, Medical Faculty, Cologne University, 50931 Cologne, Germany (C.S.C.); andHeinrich-Heine Universität Düsseldorf, 40225 Dusseldorf, Germany (R.S., S.W.-P.).ORCID http://orcid.org/0000-0002-1317-7716
Stefanie Weidtkamp-PetersBiocenter, Cologne University, Botanical Institute, 50674 Cologne, Germany (M.P., I.S., K.K., J.F.U., M.H.);Preclinical Target Development and Genomics and Proteomics Core Facilities, German Cancer Research Center, 69120 Heidelberg, Germany (M.K.);Center for Biochemistry, Institute of Biochemistry I, Medical Faculty, Cologne University, 50931 Cologne, Germany (C.S.C.); andHeinrich-Heine Universität Düsseldorf, 40225 Dusseldorf, Germany (R.S., S.W.-P.).ORCID http://orcid.org/0000-0001-7734-3771
Martin HülskampBiocenter, Cologne University, Botanical Institute, 50674 Cologne, Germany (M.P., I.S., K.K., J.F.U., M.H.);Preclinical Target Development and Genomics and Proteomics Core Facilities, German Cancer Research Center, 69120 Heidelberg, Germany (M.K.);Center for Biochemistry, Institute of Biochemistry I, Medical Faculty, Cologne University, 50931 Cologne, Germany (C.S.C.); andHeinrich-Heine Universität Düsseldorf, 40225 Dusseldorf, Germany (R.S., S.W.-P.) martin.huelskamp@uni-koeln.de.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The MBW (for R2R3MYB, basic helix-loop-helix [bHLH], and WD40) genes comprise an evolutionarily conserved gene cassette that regulates several traits such as (pro)anthocyanin and anthocyanin biosynthesis and epidermal cell differentiation in plants. Trichome differentiation in Arabidopsis (Arabidopsis thaliana) is governed by GLABRA1 (GL1; R2R3MYB), GL3 (bHLH), and transparent TESTA GLABRA1 (TTG1; WD40). They are thought to form a trimeric complex that acts as a transcriptional activation complex. We provide evidence that these three MBW proteins form either GL1 GL3 or GL3 TTG1 dimers. The formation of each dimer is counteracted by the respective third protein in yeast three-hybrid assays, pulldown experiments (luminescence-based mammalian interactome), and fluorescence lifetime imaging microscopy-fluorescence resonance energy transfer studies. We further show that two target promoters, Triptychon (TRY) and CAPRICE (CPC), are differentially regulated: GL1 represses the activation of the TRY promoter by GL3 and TTG1, and TTG1 suppresses the activation of the CPC promoter by GL1 and GL3. Our data suggest that the transcriptional activation by the MBW complex involves alternative complex formation and that the two dimers can differentially regulate downstream genes.

Indexed as

Binding, CompetitiveArabidopsisArabidopsis ProteinsBasic Helix-Loop-Helix ProteinsDNA-Binding ProteinsFluorescence Resonance Energy TransferGene Expression Regulation, PlantMicroscopy, FluorescenceModels, BiologicalPlants, Genetically ModifiedPromoter Regions, GeneticProtein BindingProtein Interaction MappingProto-Oncogene Proteins c-mybTransformation, GeneticTwo-Hybrid System TechniquesArabidopsis ProteinsBasic Helix-Loop-Helix ProteinsCPC protein, ArabidopsisDNA-Binding ProteinsGL1 protein, ArabidopsisGL3 protein, ArabidopsisProto-Oncogene Proteins c-mybTTG1 protein, Arabidopsis

Identifiers

PMID25926482
PMCPMC4453790

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.