Evidence map›Paper›PMID 25925837›Full record

ArticleStem cells translational medicine2015

Freshly thawed and continuously cultured human bone marrow-derived mesenchymal stromal cells comparably ameliorate allergic airways inflammation in immunocompetent mice.

Fernanda F Cruz, Zachary D Borg, Meagan Goodwin, Dino Sokocevic, Darcy Wagner, David H McKenna, Patricia R M Rocco, Daniel J Weiss

Registry-linked trialOpen access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cells translational medicine, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT05147688 (Safety of Cultured Allogeneic Adult Umbilical Cord Derived Mesenchymal Stem Cell Intravenous Infusion for the Treatment of Pulmonary Diseases), which is not on this map. Cited by 47 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
47citing papers in PubMed, 1 pooled it
5.2field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT05147688 phase1recruitingnot on this mapstarted 2021, after this paper: background citation

Safety of Cultured Allogeneic Adult Umbilical Cord Derived Mesenchymal Stem Cell Intravenous Infusion for the Treatment of Pulmonary Diseases

TypeinterventionalSponsorThe Foundation for Orthopaedics and Regenerative MedicineRan2021 to 2025Enrolled20ConditionsPulmonary Disease, Asthma, Chronic Obstructive Pulmonary DiseaseArmsAlloRx
3 · Its place in the literature

Who cites it

47 citing papers in PubMed, 1 synthesis or guideline pooled it, 84 citations in OpenAlex.

  1. Pooled it
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  4. Cold Chain Delivery in Cell Therapies.Advances in experimental medicine and biology · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Fernanda F CruzDepartment of Medicine, University of Vermont College of Medicine, Burlington, Vermont, USA; Laboratory of Pulmonary Investigation, Carlos Chagas Filho Biophysics Institute, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil; Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, Minnesota, USA.
Zachary D BorgDepartment of Medicine, University of Vermont College of Medicine, Burlington, Vermont, USA; Laboratory of Pulmonary Investigation, Carlos Chagas Filho Biophysics Institute, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil; Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, Minnesota, USA.
Meagan GoodwinDepartment of Medicine, University of Vermont College of Medicine, Burlington, Vermont, USA; Laboratory of Pulmonary Investigation, Carlos Chagas Filho Biophysics Institute, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil; Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, Minnesota, USA.
Dino SokocevicDepartment of Medicine, University of Vermont College of Medicine, Burlington, Vermont, USA; Laboratory of Pulmonary Investigation, Carlos Chagas Filho Biophysics Institute, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil; Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, Minnesota, USA.
Darcy WagnerDepartment of Medicine, University of Vermont College of Medicine, Burlington, Vermont, USA; Laboratory of Pulmonary Investigation, Carlos Chagas Filho Biophysics Institute, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil; Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, Minnesota, USA.
David H McKennaDepartment of Medicine, University of Vermont College of Medicine, Burlington, Vermont, USA; Laboratory of Pulmonary Investigation, Carlos Chagas Filho Biophysics Institute, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil; Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, Minnesota, USA.
Patricia R M RoccoDepartment of Medicine, University of Vermont College of Medicine, Burlington, Vermont, USA; Laboratory of Pulmonary Investigation, Carlos Chagas Filho Biophysics Institute, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil; Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, Minnesota, USA.
Daniel J WeissDepartment of Medicine, University of Vermont College of Medicine, Burlington, Vermont, USA; Laboratory of Pulmonary Investigation, Carlos Chagas Filho Biophysics Institute, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil; Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, Minnesota, USA dweiss@uvm.edu.
University of Vermont · USUniversidade Federal do Rio de Janeiro · BRUniversity of Minnesota · US

Funding

Women's CancerP30CA077598 · NCI · UNIVERSITY OF MINNESOTA TWIN CITIES · PI Douglas Yee · 1998 to 2026
$100.4M
WEIGHT LOSS & ASTHMAP20RR015557 · NCRR · UNIVERSITY OF VERMONT &ST AGRIC COLLEGE · PI RINCON, MERCEDES · 2000 to 2009
$21.4M
PREPARATION AND DISTRUBUTION OF ADULT STEM CELLS: HUMAN &RODENTP40RR017447 · NCRR · TULANE UNIVERSITY OF LOUISIANA · PI PROCKOP, DARWIN JOHNSON · 2003 to 2011
$8.9M
Bioengineering New Lungs from Cadaveric Lung ScaffoldsRC4HL106625 · NHLBI · UNIVERSITY OF VERMONT & ST AGRIC COLLEGE · PI WEISS, DANIEL J · 2010 to 2010
$4.3M
De-Cellularized Human Lungs for Ex Vivo Lung RegenerationR21HL108689 · NHLBI · UNIVERSITY OF VERMONT & ST AGRIC COLLEGE · PI WEISS, DANIEL J · 2011 to 2012
$398k
NCI NIH HHS P30 CA077598NCRR NIH HHS P20 RR015557NCRR NIH HHS P20RR15557NCRR NIH HHS P40 RR017447NHLBI NIH HHS HHSN268201000008CNHLBI NIH HHS R21 HL108689NHLBI NIH HHS R21HL108689NHLBI NIH HHS RC4 HL106625NHLBI NIH HHS RC4HL106625PHS HHS HHSN26820100008C
6 · The paper itself

Abstract

unlabelledRecent data suggest that freshly thawed previously frozen mesenchymal stromal cells (MSCs) may not have the same effectiveness or breadth of anti-inflammatory activities as do continuously cultured MSCs. This has significant implications for clinical use, in which many infusion schemes use frozen cells thawed at the bedside for administration. The available data, however, predominantly evaluate in vitro MSC properties, and so far there has been limited in vivo analysis. To further assess this issue, we compared freshly thawed (thawed) versus continuously cultured (fresh) human bone marrow-derived MSC (hMSC) administration in a mouse model of mixed Th2/Th17 allergic airway inflammation induced by Aspergillus hyphal extract (AHE) exposures in immunocompetent C57Bl/6 mice. Control cell populations included fresh versus thawed murine bone marrow-derived MSCs (mMSCs) and human lung fibroblasts (HLFs). Systemic administration of both thawed and fresh hMSCs and mMSCs, but not HLFs, at the onset of antigen challenge in previously sensitized mice significantly ameliorated the AHE-provoked increases in airway hyper-reactivity, lung inflammation, and antigen-specific CD4 T-cell Th2 and Th17 phenotype. Notably, there was no difference in effects of fresh versus thawed hMSCs or mMSCs on any outcome measured except for some variability in the effects on the bronchoalveolar lavage fluid composition. These results demonstrated potent xenogeneic effects of human MSCs in an immunocompetent mouse model of allergic airways inflammation and that thawed MSCs are as effective as fresh MSCs. The question of fresh versus thawed MSC effectiveness needs to be investigated carefully and may differ in different in vivo disease-specific models. SIGNIFICANCE: This study addressed whether freshly thawed mesenchymal stromal cells (MSCs) are as effective in in vivo settings as those that have been continuously cultured. It also provided further data demonstrating that xenogeneic use of MSCs in immunocompetent mice is as effective as murine MSCs. This information provides further support and direction for potential clinical use of MSCs in patients with severe asthma.

Indexed as

Mesenchymal Stem Cell TransplantationAnimalsAsthmaBone Marrow CellsCryopreservationDisease Models, AnimalHeterograftsHumansMesenchymal Stem CellsMiceTh17 CellsTh2 CellsAsthmaCryopreservationMesenchymal stromal cellsMouse

Identifiers

PMID25925837
PMCPMC4449099
OpenAlexW2145815234

What OpenQuestion holds

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Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.