ArticleEMBO reports2015
Paternal H3K4 methylation is required for minor zygotic gene activation and early mouse embryonic development.
Article in EMBO reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
40 citing papers in PubMed, 80 citations in OpenAlex.
- Clinical Implications of Paternal Age in Assisted Reproduction: Integrating Sperm Epigenetic Evidence.Journal of clinical medicine · 2026Review
- Epigenetic remodeling during early embryonic development.Frontiers in cell and developmental biology · 2026Review
- Pre-marking chromatin with H3K4 methylation is required for accurate zygotic genome activation and development.Nature communications · 2025Article
- A father's legacy: the sperm epigenome, preimplantation development, and paternal environment.Epigenomics · 2025Review
- Epigenome dynamics in early mammalian embryogenesis.Nature reviews. Genetics · 2025Review
- Paternal Exposure to Methylphenidate Induces Poor-Quality Blastocyst and Epigenetic Changes.Molecular reproduction and development · 2025Article
- Acetylation at lysine 27 on maternal H3.3 regulates minor zygotic genome activation.Cell reports · 2025Article
- DNA methylation modulates nucleosome retention in sperm and H3K4 methylation deposition in early mouse embryos.Nature communications · 2025Article
- A vector system encoding histone H3 mutants facilitates manipulations of the neuronal epigenome.Scientific reports · 2024Article
- RNA helicase DEAD-box-5 is involved in R-loop dynamics of preimplantation embryos.Animal bioscience · 2024Article
- The Dynamics of Histone Modifications during Mammalian Zygotic Genome Activation.International journal of molecular sciences · 2024Review
- The Association between Long-Term DDT or DDE Exposures and an Altered Sperm Epigenome-a Cross-Sectional Study of Greenlandic Inuit and South African VhaVenda Men.Environmental health perspectives · 2024Article
- When Dad's Stress Gets under Kid's Skin-Impacts of Stress on Germline Cargo and Embryonic Development.Biomolecules · 2023Review
- Leveraging dominant-negative histone H3 K-to-M mutations to study chromatin during differentiation and development.Development (Cambridge, England) · 2023Review
- Emerging evidence that the mammalian sperm epigenome serves as a template for embryo development.Nature communications · 2023Review
- Dynamic changes of histone methylation in mammalian oocytes and early embryos.Histochemistry and cell biology · 2022Review
- Nutritional Status Impacts Epigenetic Regulation in Early Embryo Development: A Scoping Review.Advances in nutrition (Bethesda, Md.) · 2021Article
- Symmetrically dimethylated histone H3R2 promotes global transcription during minor zygotic genome activation in mouse pronuclei.Scientific reports · 2021Article
- Sperm histone H3 lysine 4 trimethylation is altered in a genetic mouse model of transgenerational epigenetic inheritance.Nucleic acids research · 2020Article
- Histone H3 methylation orchestrates transcriptional program in mouse spermatogenic cell line.The Journal of reproduction and development · 2020Article
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Epigenetic modifications, such as DNA methylation and histone modifications, are dynamically altered predominantly in paternal pronuclei soon after fertilization. To identify which histone modifications are required for early embryonic development, we utilized histone K-M mutants, which prevent endogenous histone methylation at the mutated site. We prepared four single K-M mutants for histone H3.3, K4M, K9M, K27M, and K36M, and demonstrate that overexpression of H3.3 K4M in embryos before fertilization results in developmental arrest, whereas overexpression after fertilization does not affect the development. Furthermore, loss of H3K4 methylation decreases the level of minor zygotic gene activation (ZGA) predominantly in the paternal pronucleus, and we obtained similar results from knockdown of the H3K4 methyltransferase Mll3/4. We therefore conclude that H3K4 methylation, likely established by Mll3/4 at the early pronuclear stage, is essential for the onset of minor ZGA in the paternal pronucleus, which is necessary for subsequent preimplantation development in mice.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.