ArticleBMC microbiology2015
Characterization of motility and piliation in pathogenic Neisseria.
Article in BMC microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- The role of extracellular DNA inJournal of bacteriology · 2026Review
- The stoichiometry of minor-to-major pilins regulates the dynamic activity of the type IVa competence pilus in Vibrio cholerae.PLoS genetics · 2026Article
- An in vitro human vessel model to studyeLife · 2025Article
- Proteins associated with environmental survival of the pathogenEpidemiology and infection · 2025Article
- The role of PilU in the surface behaviors ofmLife · 2025Article
- Architectural dissection of adhesive bacterial cell surface appendages from a "molecular machines" viewpoint.Journal of bacteriology · 2024Review
- Type IV pili trigger episymbiotic association of Saccharibacteria with its bacterial host.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Neisseria genes required for persistence identified via in vivo screening of a transposon mutant library.PLoS pathogens · 2022Article
- Antibacterial Activity of Silver and Gold Particles Formed on Titania Thin Films.Nanomaterials (Basel, Switzerland) · 2022Article
- External Stresses Affect Gonococcal Type 4 Pilus Dynamics.Frontiers in microbiology · 2022Article
- Article
- RpoN and the Nps and Npa two-component regulatory system control pilE transcription in commensal Neisseria.MicrobiologyOpen · 2019Article
- Genus-Wide Comparative Genomics Analysis ofInternational journal of genomics · 2019Article
- Cargo transport shapes the spatial organization of a microbial community.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- A null model for microbial diversification.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Host cell-derived lactate functions as an effector molecule in Neisseria meningitidis microcolony dispersal.PLoS pathogens · 2017Article
- Neisseria meningitidis Polynucleotide Phosphorylase Affects Aggregation, Adhesion, and Virulence.Infection and immunity · 2016Article
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe type IV pili (Tfp) of pathogenic Neisseria (i.e., N. gonorrhoeae and N. meningitidis) are essential for twitching motility. Tfp retraction, which is dependent on the ATPase PilT, generates the forces that move bacteria over surfaces. Neisseria motility has mainly been studied in N. gonorrhoeae whereas the motility of N. meningitidis has not yet been characterized.
resultsIn this work, we analyzed bacterial motility and monitored Tfp retraction using live-cell imaging of freely moving bacteria. We observed that N. meningitidis moved over surfaces at an approximate speed of 1.6 μm/s, whereas N. gonorrhoeae moved with a lower speed (1.0 μm/s). An alignment of the meningococcal and gonococcal pilT promoters revealed a conserved single base pair variation in the -10 promoter element that influence PilT expression. By tracking mutants with altered pilT expression or pilE sequence, we concluded that the difference in motility speed was independent of both. Live-cell imaging using total internal reflection fluorescence microscopy demonstrated that N. gonorrhoeae more often moved with fewer visible retracting filaments when compared to N. meningitidis. Correspondingly, meningococci also displayed a higher level of piliation in transmission electron microscopy. Nevertheless, motile gonococci that had the same number of filaments as N. meningitidis still moved with a lower speed.
conclusionsThese data reveal differences in both speed and piliation between the pathogenic Neisseria species during twitching motility, suggesting a difference in Tfp-dynamics.
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