Evidence map›Paper›PMID 25924612›Full record

ArticleBMC biochemistry2015

Sigma-1 receptor directly interacts with Rac1-GTPase in the brain mitochondria.

Nino Natsvlishvili, Nino Goguadze, Elene Zhuravliova, David Mikeladze

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC biochemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.

0numbers the graph read from it
0cells of the map it votes in
45citing papers in PubMed
3.0field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

45 citing papers in PubMed, 68 citations in OpenAlex.

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  17. PTSD as an Endothelial Disease: Insights From COVID-19.Frontiers in cellular neuroscience · 2021
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Nino NatsvlishviliInstitute of Chemical Biology, Ilia State University, 3/5 Cholokashvili av, Tbilisi, 0162, Georgia. nino_natsvlishvili@iliauni.edu.ge.
Nino GoguadzeInstitute of Chemical Biology, Ilia State University, 3/5 Cholokashvili av, Tbilisi, 0162, Georgia. nino.goguadze.2@iliauni.edu.ge.
Elene ZhuravliovaInstitute of Chemical Biology, Ilia State University, 3/5 Cholokashvili av, Tbilisi, 0162, Georgia. elene_zhuravliova@iliauni.edu.ge.
David MikeladzeInstitute of Chemical Biology, Ilia State University, 3/5 Cholokashvili av, Tbilisi, 0162, Georgia. davit_mikeladze@iliauni.edu.ge.
Ilia State University · GE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundSmall Rho-GTPases are critical mediators of neuronal plasticity and are involved in the pathogenesis of several psychiatric and neurological disorders. Rac-GTPase forms a multiprotein complex with upstream and downstream regulators that are essential for the spatiotemporal transmission of Rac signaling. The sigma-1 receptor (Sig1R) is a ligand-regulated membrane protein chaperone, and multiprotein complex assembly is essential to sigma-receptor function.

resultsUsing immunoprecipitation techniques, we have shown that in mitochondrial membranes Sig1R could directly interact with Rac1. Besides Rac1, the Sig1R forms complexes with inositol 1,4,5-trisphosphate receptor and Bcl2, suggesting that mitochondrial associated membranes (MAM) are involved in this macromolecular complex formation. Assembly of this complex is ligand-specific and depends on the presence of sigma agonist/antagonist, as well as on the presence of GTP/GDP. Treatment of mitochondrial membranes with (+)-pentazocine leads to the (+)-pentazocine-sensitive phosphorylation of Bad and the pentazocine-sensitive NADPH-dependent production of ROS.

conclusionWe suggest that Sig1R through Rac1 signaling induces mild oxidative stress that possibly is involved in the regulation of neuroplasticity, as well as in the prevention of apoptosis and autophagy.

Indexed as

AnimalsBrainCattleEndoplasmic Reticulum Chaperone BiPHeat-Shock ProteinsInositol 1,4,5-Trisphosphate ReceptorsMitochondriaProtein BindingProto-Oncogene Proteins c-bcl-2rac1 GTP-Binding ProteinReceptors, sigmaSigma-1 ReceptorEndoplasmic Reticulum Chaperone BiPHeat-Shock ProteinsInositol 1,4,5-Trisphosphate ReceptorsProto-Oncogene Proteins c-bcl-2rac1 GTP-Binding ProteinReceptors, sigmaSigma-1 Receptor

Identifiers

PMID25924612
PMCPMC4430930
OpenAlexW2055610325

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.