ArticleGenome biology2015
Cloning-free CRISPR/Cas system facilitates functional cassette knock-in in mice.
Article in Genome biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 179 papers.
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Who cites it
179 citing papers in PubMed, 314 citations in OpenAlex.
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- DNA repair protein RAD50 is involved in the streptozotocin-induced diabetes susceptibility of mice.Experimental animals · 2025Article
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- Improved protocol for the vitrification and warming of rat zygotes by optimizing the warming solution and oocyte donor age.PloS one · 2025Article
- Effects of centrifugation treatment before electroporation on gene editing in pig embryos.In vitro cellular & developmental biology. Animal · 2024Article
119 more citing papers are in PubMed but not listed here.
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Authors and funding
10 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although the CRISPR/Cas system has enabled one-step generation of knockout mice, low success rates of cassette knock-in limit its application range. Here we show that cloning-free, direct nuclear delivery of Cas9 protein complex with chemically synthesized dual RNAs enables highly efficient target digestion, leading to generation of knock-in mice carrying a functional cassette with up to 50% efficiency, compared with just 10% by a commonly used method consisting of Cas9 mRNA and single guide RNA. Our cloning-free CRISPR/Cas system facilitates rapid one-step generation of cassette knock-in mice, accelerating functional genomic research by providing various in vivo genetic tools.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.