Evidence map›Paper›PMID 25924224›Full record

ReviewBiomolecules2015

Structural and biochemical investigation of bacteriophage N4-encoded RNA polymerases.

Bryan R Lenneman, Lucia B Rothman-Denes

Abstract readReview
In one paragraph

Review in Biomolecules, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.

0numbers the graph read from it
0cells of the map it votes in
33citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

33 citing papers in PubMed.

  1. Article
  2. Article
  3. The biology of jumbo phages.Nature communications · 2026
    Review
  4. Article
  5. An N4-likeJournal of bacteriology · 2026
    Article
  6. Phage N4 uses a SAR endolysin-holin system for host cell lysis.bioRxiv : the preprint server for biology · 2025
    Article
  7. Article
  8. An N4-likebioRxiv : the preprint server for biology · 2025
    Article
  9. Article
  10. Article
  11. Viral Genome Delivery Across Bacterial Cell Surfaces.Annual review of microbiology · 2024
    Review
  12. Article
  13. Integrative structural analysis ofResearch square · 2024
    Article
  14. Review
  15. Article
  16. Article
  17. Article
  18. International journal of molecular sciences · 2022
    Article
  19. Development of ONT-cappable-seq to unravel the transcriptional landscape ofComputational and structural biotechnology journal · 2022
    Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Bryan R LennemanCommittee on Genetics, Genomics, and Systems Biology, The University of Chicago, 920 East 58th Street, Chicago, IL 60637, USA. blenneman@uchicago.edu.
Lucia B Rothman-DenesCommittee on Genetics, Genomics, and Systems Biology, The University of Chicago, 920 East 58th Street, Chicago, IL 60637, USA. lbrd@uchicago.edu.

Funding

PREDOCTORAL TRAINING IN GENETICS AND REGULATIONT32GM007197 · NIGMS · UNIVERSITY OF CHICAGO · PI ROTHMAN-DENES, LUCIA B. B · 1985 to 2020
$17.0M
Transcribing Activities in N4 Infected E. ColiR01AI012575 · NIAID · UNIVERSITY OF CHICAGO · PI ROTHMAN-DENES, LUCIA B. B · 1985 to 2013
$7.0M
NIAID NIH HHS R01 AI012575NIAID NIH HHS R01 AI-12575NIGMS NIH HHS T32 GM007197
6 · The paper itself

Abstract

Bacteriophage N4 regulates the temporal expression of its genome through the activity of three distinct RNA polymerases (RNAP). Expression of the early genes is carried out by a phage-encoded, virion-encapsidated RNAP (vRNAP) that is injected into the host at the onset of infection and transcribes the early genes. These encode the components of new transcriptional machinery (N4 RNAPII and cofactors) responsible for the synthesis of middle RNAs. Both N4 RNAPs belong to the T7-like "single-subunit" family of polymerases. Herein, we describe their mechanisms of promoter recognition, regulation, and roles in the phage life cycle.

Indexed as

Amino Acid SequenceBacteriophage N4Catalytic DomainDNA-Directed RNA PolymerasesMolecular Sequence DataProtein BindingTranscriptional ActivationViral ProteinsDNA-Directed RNA PolymerasesViral Proteins

Identifiers

PMID25924224
PMCPMC4496689

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.