ArticleCellular microbiology2015
Atg8 is involved in endosomal and phagosomal acidification in the parasitic protist Entamoeba histolytica.
Article in Cellular microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 19 citations in OpenAlex.
- Entamoeba histolytica clones presenting transient virulent phenotypes: subsequent fate of forcibly induced virulent clones by hamster's liver abscess model.Gut pathogens · 2026Article
- A multifunctional organelle coordinates phagocytosis and chlorophagy in a marine eukaryote phytoplankton Scyphosphaera apsteinii.The New phytologist · 2025Article
- Encystation and Stress Responses under the Control of Ubiquitin-like Proteins in Pathogenic Amoebae.Microorganisms · 2023Review
- Non-Vesicular Lipid Transport Machinery inInternational journal of molecular sciences · 2023Review
- Different Drugs, Same End: Ultrastructural Hallmarks of Autophagy in Pathogenic Protozoa.Frontiers in microbiology · 2022Review
- Proteomic analysis of Atg8-dependent recruitment of phagosomal proteins in the enteric protozoan parasiteFrontiers in cellular and infection microbiology · 2022Article
- Anaerobic peroxisomes in Entamoeba histolytica metabolize myo-inositol.PLoS pathogens · 2021Article
- Trogocytosis in Unicellular Eukaryotes.Cells · 2021Review
- The Autophagy Machinery in Human-Parasitic Protists; Diverse Functions for Universally Conserved Proteins.Cells · 2021Review
- Rab7D small GTPase is involved in phago-, trogocytosis and cytoskeletal reorganization in the enteric protozoan Entamoeba histolytica.Cellular microbiology · 2021Article
- The Dichloromethane Fraction ofFrontiers in cellular and infection microbiology · 2021Article
- The dynamics of ultrastructural changes duringParasitology · 2020Article
- Two isotypes of phosphatidylinositol 3-phosphate-binding sorting nexins play distinct roles in trogocytosis in Entamoeba histolytica.Cellular microbiology · 2020Article
- Ubiquitin-like Atg8 protein is expressed during autophagy and the encystation process in Naegleria gruberi.Parasitology research · 2017Article
- Changes in 30K protein synthesis during delayed degeneration of the silk gland by a caspase-dependent pathway in a Bombyx (silkworm) mutant.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2016Article
- Atg8 is involved in endosomal and phagosomal acidification in the parasitic protist Entamoeba histolytica.Cellular microbiology · 2015Article
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Authors and funding
7 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Autophagy is one of two major bulk protein degradation systems and is conserved throughout eukaryotes. The protozoan Entamoeba histolytica, which is a human intestinal parasite, possesses a restricted set of autophagy-related (Atg) proteins compared with other eukaryotes and thus represents a suitable model organism for studying the minimal essential components and ancestral functions of autophagy. E. histolytica possesses two conjugation systems: Atg8 and Atg5/12, although a gene encoding Atg12 is missing in the genome. Atg8 is considered to be the central and authentic marker of autophagosomes, but recent studies have demonstrated that Atg8 is not exclusively involved in autophagy per se, but other fundamental mechanisms of vesicular traffic. To investigate this question in E. histolytica, we studied on Atg8 during the proliferative stage. Atg8 was constitutively expressed in both laboratory-maintained and recently established clinical isolates and appeared to be lipid-modified in logarithmic growth phase, suggesting a role of Atg8 in non-stress and proliferative conditions. These findings are in contrast to those for Entamoeba invadens, in which autophagy is markedly induced during an early phase of differentiation from the trophozoite into the cyst. The repression of Atg8 gene expression in En. histolytica by antisense small RNA-mediated transcriptional gene silencing resulted in growth retardation, delayed endocytosis and reduced acidification of endosomes and phagosomes. Taken together, these results suggest that Atg8 and the Atg8 conjugation pathway have some roles in the biogenesis of endosomes and phagosomes in this primitive eukaryote.
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