ArticlePloS one2015
Blastocystis Isolates from Patients with Irritable Bowel Syndrome and from Asymptomatic Carriers Exhibit Similar Parasitological Loads, but Significantly Different Generation Times and Genetic Variability across Multiple Subtypes.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 35 citations in OpenAlex.
- From Bioinformatic Modeling to Clinical Observation: Potential Implications of Ribosomal RNA Folding inPathogens (Basel, Switzerland) · 2025Article
- Subtype-host patterns and genetic differentiation ofHeliyon · 2024Article
- Irritable Bowel Syndrome Associated with Blastocystis hominis or Without Relationship to It? A Case-Control Study and Minireview.Acta parasitologica · 2024Review
- Identification of Blastocystis sp. in school children from a rural Mexican village: subtypes and risk factors analysis.Parasitology research · 2023Observational
- Levels of Genetic Variants Among Symptomatic Blastocystis Subtypes and their Relationship to Mucosal Immune Surveillance in the Precancerous Colons of Experimentally Infected Rats.Acta parasitologica · 2023Article
- An update on the distribution ofHeliyon · 2022Article
- Gut Microbiota Shifting in Irritable Bowel Syndrome: The Mysterious Role ofFrontiers in medicine · 2022Review
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- Prevalence and subtypes of Blastocystis among migrant workers from different working sectors in Peninsular Malaysia.Parasitology research · 2020Article
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- Molecular genotyping of Blastocystis spp. in wild mammals from Mexico.Parasitology research · 2020Article
- Use of next-generation amplicon sequencing to study Blastocystis genetic diversity in a rural human population from Mexico.Parasites & vectors · 2019Article
- Intestinal parasitic infections and associated factors in children of three rural schools in Colombia. A cross-sectional study.PloS one · 2019Article
- Is the genetic variability of Cathepsin B important in the pathogenesis of Blastocystis spp.?Parasitology research · 2018Article
- Can the pyruvate: ferredoxin oxidoreductase (PFOR) gene be used as an additional marker to discriminate among Blastocystis strains or subtypes?Parasites & vectors · 2018Article
- Genetic Diversity and Distribution ofBioMed research international · 2018Article
- Distribution of Blastocystis subtypes isolated from humans from an urban community in Rio de Janeiro, Brazil.Parasites & vectors · 2017Article
- Clarifying the Cryptic Host Specificity of Blastocystis spp. Isolates from Alouatta palliata and A. pigra Howler Monkeys.PloS one · 2017Article
- Comprehensive Study of Phenotypic and Growth Rate Features ofIranian journal of parasitologyArticle
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Authors and funding
13 authors at 6 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Blastocystis spp is a common intestinal parasite of humans and animals that has been associated to the etiology of irritable bowel syndrome (IBS); however, some studies have not found this association. Furthermore, many biological features of Blastocystis are little known. The objective of present study was to assess the generation times of Blastocystis cultures, from IBS patients and from asymptomatic carriers. A total of 100 isolates were obtained from 50 IBS patients and from 50 asymptomatic carriers. Up to 50 mg of feces from each participant were cultured in Barret's and in Pavlova's media during 48 h. Initial and final parasitological load were measured by microscopy and by quantitative PCR. Amplicons were purified, sequenced and submitted to GenBank; sequences were analysed for genetic diversity and a Bayesian inference allowed identifying genetic subtypes (ST). Generation times for Blastocystis isolates in both media, based on microscopic measures and molecular assays, were calculated. The clinical symptoms of IBS patients and distribution of Blastocystis ST 1, 2 and 3 in both groups was comparable to previous reports. Interestingly, the group of cases showed scarce mean nucleotide diversity (π) as compared to the control group (0.011±0.016 and 0.118±0.177, respectively), whilst high gene flow and small genetic differentiation indexes between different ST were found. Besides, Tajima's D test showed negative values for ST1-ST3. No statistical differences regarding parasitological load between cases and controls in both media, as searched by microscopy and by qPCR, were detected except that parasites grew faster in Barret's than in Pavlova's medium. Interestingly, slow growth of isolates recovered from cases in comparison to those of controls was observed (p<0.05). We propose that generation times of Blastocystis might be easily affected by intestinal environmental changes due to IBS probably because virulent strains with slow growth may be selected, reducing their genetic variability.
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