Evidence map›Paper›PMID 25923693›Full record

ArticlePloS one2015

Organelle-Specific Nitric Oxide Detection in Living Cells via HaloTag Protein Labeling.

Jianhua Wang, Yuzheng Zhao, Chao Wang, Qian Zhu, Zengmin Du, Aiguo Hu, Yi Yang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Illuminating NADDevelopmental cell · 2020
    Article
  5. Article
  6. Review
  7. Article
  8. Gasotransmitter Heterocellular Signaling.Antioxidants & redox signaling · 2017
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Jianhua WangSynthetic Biology and Biotechnology Laboratory, State Key Laboratory of Bioreactor Engineering, CAS Center for Excellence in Brain Science, School of Pharmacy, East China University of Science and Technology, 130 Mei Long Road, Shanghai, 200237, P. R. China.
Yuzheng ZhaoSynthetic Biology and Biotechnology Laboratory, State Key Laboratory of Bioreactor Engineering, CAS Center for Excellence in Brain Science, School of Pharmacy, East China University of Science and Technology, 130 Mei Long Road, Shanghai, 200237, P. R. China.
Chao WangShanghai Key Laboratory of Advanced Polymeric Materials, School of Materials Science and Engineering, East China University of Science and Technology, 130 Mei Long Road, Shanghai, 200237, P. R. China.
Qian ZhuSynthetic Biology and Biotechnology Laboratory, State Key Laboratory of Bioreactor Engineering, CAS Center for Excellence in Brain Science, School of Pharmacy, East China University of Science and Technology, 130 Mei Long Road, Shanghai, 200237, P. R. China.
Zengmin DuSynthetic Biology and Biotechnology Laboratory, State Key Laboratory of Bioreactor Engineering, CAS Center for Excellence in Brain Science, School of Pharmacy, East China University of Science and Technology, 130 Mei Long Road, Shanghai, 200237, P. R. China.
Aiguo HuShanghai Key Laboratory of Advanced Polymeric Materials, School of Materials Science and Engineering, East China University of Science and Technology, 130 Mei Long Road, Shanghai, 200237, P. R. China.
Yi YangSynthetic Biology and Biotechnology Laboratory, State Key Laboratory of Bioreactor Engineering, CAS Center for Excellence in Brain Science, School of Pharmacy, East China University of Science and Technology, 130 Mei Long Road, Shanghai, 200237, P. R. China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Nitric oxide (NO) is a membrane-permeable signaling molecule that is constantly produced, transferred, and consumed in vivo. NO participates and plays important roles in multiple biological processes. However, spatiotemporal imaging of NO in living cells is challenging. To fill the gap in currently used techniques, we exploited the versatility of HaloTag technology and synthesized a novel organelle-targetable fluorescent probe called HTDAF-2DA. We demonstrate the utility of the probe by monitoring subcellular NO dynamics. The developed strategy enables precise determination of local NO function.

Indexed as

AlkylationBacteriaCell SurvivalChloridesFluorescent DyesHeLa CellsHepatocytesHumansHydrolasesMCF-7 CellsMicroscopy, FluorescenceNitric OxideOptical ImagingOrganellesStaining and LabelingChloridesFluorescent Dyeshaloalkane dehalogenaseHydrolasesNitric Oxide

Identifiers

PMID25923693
PMCPMC4414533

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.