ArticlePloS one2015
Slow freezing, but not vitrification supports complete spermatogenesis in cryopreserved, neonatal sheep testicular xenografts.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 72 citations in OpenAlex.
- Slow freezing and 3DAnimal reproduction · 2026Article
- Testicular tissue transplantation: from animal models to clinical application.Human reproduction open · 2026Review
- Testicular cryopreservation: From technical aspects to practical applications.Histology and histopathology · 2025Review
- Evaluation of Different Cryoprotectant Combinations in Testicular Vitrification in Dogs.Reproduction in domestic animals = Zuchthygiene · 2025Article
- Solid Surface Vitrification Is Better than Slow Freezing for the Long-Term Preservation of Testicular Fragments from Prepubertal Collared Peccaries (Animals : an open access journal from MDPI · 2025Article
- Influence of Three Combinations of Cryoprotectants and Two Warming Temperatures on Cellular Morphology, Morphometry and Mitochondrial Activity of Vitrified Canine Testicles.Reproduction in domestic animals = Zuchthygiene · 2025Article
- Cryopreservation and culture strategies for testicular tissue and cells in small and large animals.Frontiers in veterinary science · 2025Review
- Review
- Advancements in fertility preservation strategies for pediatric male cancer patients: a review of cryopreservation and transplantation of immature testicular tissue.Reproductive biology and endocrinology : RB&E · 2024Review
- Testicular Tissue Vitrification: a Promising Strategy for Male Fertility Preservation.Reproductive sciences (Thousand Oaks, Calif.) · 2023Review
- Fertility preservation in pediatric healthcare: a review.Frontiers in endocrinology · 2023Review
- Biomaterials for Testicular Bioengineering: How far have we come and where do we have to go?Frontiers in endocrinology · 2023Review
- Cryopreservation of African painted dog (Frontiers in veterinary science · 2023Article
- Cryopreservation of Testicular Tissue from Adult Red-Rumped Agoutis (Animals : an open access journal from MDPI · 2022Article
- Canine and Feline Testicular Preservation.Animals : an open access journal from MDPI · 2022Review
- DNA integrity and viability of testicular cells from diverse wild species after slow freezing or vitrification.Frontiers in veterinary science · 2022Article
- Current scenario and challenges ahead in application of spermatogonial stem cell technology in livestock.Journal of assisted reproduction and genetics · 2021Review
- Strategies for cryopreservation of testicular cells and tissues in cancer and genetic diseases.Cell and tissue research · 2021Review
- Review
- Three-dimensional testicular organoids as novelEnvironmental epigenetics · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ability to spur growth of early stage gametic cells recovered from neonates could lead to significant advances in rescuing the genomes of rare genotypes or endangered species that die unexpectedly. The purpose of this study was to determine, for the first time, the ability of two substantially different cryopreservation approaches, slow freezing versus vitrification, to preserve testicular tissue of the neonatal sheep and subsequently allow initiation of spermatogenesis post-xenografting. Testis tissue from four lambs (3-5 wk old) was processed and then untreated or subjected to slow freezing or vitrification. Tissue pieces (fresh, n = 214; slow freezing, then thawing, n = 196; vitrification, then warming, n = 139) were placed subcutaneously under the dorsal skin of SCID mice and then grafts recovered and evaluated 17 wk later. Grafts from fresh and slow frozen tissue contained the most advanced stages of spermatogenesis, including normal tubule architecture with elongating spermatids in ~1% (fresh) and ~10% (slow frozen) of tubules. Fewer than 2% of seminiferous tubules advanced to the primary spermatocyte stage in xenografts derived from vitrified tissue. Results demonstrate that slow freezing of neonatal lamb testes was far superior to vitrification in preserving cellular integrity and function after xenografting, including allowing ~10% of tubules to retain the capacity to resume spermatogenesis and yield mature spermatozoa. Although a first for any ruminant species, findings also illustrate the importance of preemptive studies that examine cryo-sensitivity of testicular tissue before attempting this type of male fertility preservation on a large scale.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.