ArticleScientific reports2015
Disruption of the rice nitrate transporter OsNPF2.2 hinders root-to-shoot nitrate transport and vascular development.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 142 citations in OpenAlex.
- Breeding for Resilient Cereals: Integrating Molecular Networks and Trait-Based Selection to Improve Nitrogen and Water Use Efficiencies.Plant, cell & environment · 2026Review
- Genetically Encoded Fluorescent Biosensors Enable Noninvasive Real-Time Visualization of Nitrate Dynamics in Intact Living Plants.Biosensors · 2026Article
- Functional characterization of transporter genes in rice node at single-cell resolution through multi-omics technologies.Genome biology · 2026Article
- Functional characterization of NRT1/PTR FAMILY transporters: looking for a needle in a haystack.The New phytologist · 2026Review
- Transcriptome-wide identification and expression analysis of lncRNAs involved in the nitrate response in wheat.BMC plant biology · 2025Article
- Optimizing Nutrient Dynamics for Crop Resilience to Abiotic Stress: An Endogenous Phytohormone Perspective.Plants (Basel, Switzerland) · 2025Review
- Single-cell transcriptome sequencing reveals the sphingolipid metabolism pathway plays an important role in leaf senescence in tobacco.BMC plant biology · 2025Article
- Identification of Advantaged Genes for Low-Nitrogen-Tolerance-Related Traits in Rice Using a Genome-Wide Association Study.International journal of molecular sciences · 2025Article
- Regulatory network of ammonium and nitrate uptake and utilization in rice.Frontiers in plant science · 2025Review
- Genome-wide identification, evolution and expression analysis unveil the role ofPhysiology and molecular biology of plants : an international journal of functional plant biology · 2024Article
- MaizeInternational journal of molecular sciences · 2023Article
- Genome-Wide Identification and Functional Analysis of Nitrate Transporter Genes (International journal of molecular sciences · 2023Article
- Review
- Nitrate-responsive OsMADS27 promotes salt tolerance in rice.Plant communications · 2023Article
- Transcriptome and Co-Expression Network Analysis Reveals the Molecular Mechanism of Rice Root Systems in Response to Low-Nitrogen Conditions.International journal of molecular sciences · 2023Article
- Unlocking the potentials of nitrate transporters at improving plant nitrogen use efficiency.Frontiers in plant science · 2023Review
- Genome-wide association study using specific-locus amplified fragment sequencing identifies new genes influencing nitrogen use efficiency in rice landraces.Frontiers in plant science · 2023Article
- Improving Nitrogen Use Efficiency in Rice for Sustainable Agriculture: Strategies and Future Perspectives.Life (Basel, Switzerland) · 2022Review
- The nitrate transporter OsNPF7.9 mediates nitrate allocation and the divergent nitrate use efficiency between indica and japonica rice.Plant physiology · 2022Article
- Transporters and transcription factors gene families involved in improving nitrogen use efficiency (NUE) and assimilation in rice (Oryza sativa L.).Transgenic research · 2022Review
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plants have evolved to express some members of the nitrate transporter 1/peptide transporter family (NPF) to uptake and transport nitrate. However, little is known of the physiological and functional roles of this family in rice (Oryza sativa L.). Here, we characterized the vascular specific transporter OsNPF2.2. Functional analysis using cDNA-injected Xenopus laevis oocytes revealed that OsNPF2.2 is a low-affinity, pH-dependent nitrate transporter. Use of a green fluorescent protein tagged OsNPF2.2 showed that the transporter is located in the plasma membrane in the rice protoplast. Expression analysis showed that OsNPF2.2 is nitrate inducible and is mainly expressed in parenchyma cells around the xylem. Disruption of OsNPF2.2 increased nitrate concentration in the shoot xylem exudate when nitrate was supplied after a deprivation period; this result suggests that OsNPF2.2 may participate in unloading nitrate from the xylem. Under steady-state nitrate supply, the osnpf2.2 mutants maintained high levels of nitrate in the roots and low shoot:root nitrate ratios; this observation suggests that OsNPF2.2 is involved in root-to-shoot nitrate transport. Mutation of OsNPF2.2 also caused abnormal vasculature and retarded plant growth and development. Our findings demonstrate that OsNPF2.2 can unload nitrate from the xylem to affect the root-to-shoot nitrate transport and plant development.
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