ArticlePloS one2015
The hemiptera (insecta) of Canada: constructing a reference library of DNA barcodes.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 101 citations in OpenAlex.
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- Exploring genetic diversity and phylogenetic connections of tropical bed bugs, Cimex hemipterus (F.) infestation in Indonesia.PloS one · 2025Article
- Principles for Constructing DNA Barcode Reference Libraries.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Building a DNA barcode reference collection of Hymenoptera in New Zealand.Biodiversity data journal · 2024Article
- The Past, Present, and Future of Wheat Dwarf Virus Management-A Review.Plants (Basel, Switzerland) · 2023Review
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- Assessment of the DNA barcode libraries for the study of the poorly-known rove beetle (Staphylinidae) fauna of West Siberia.Biodiversity data journal · 2023Article
- The genusZooKeys · 2023Article
- Molecular characterization and phylogenetic relationships amongSaudi journal of biological sciences · 2022Article
- Diversity and Molecular Barcoding of Stink Bugs (Hemiptera: Pentatomidae) Associated with Macadamia in South Africa.Insects · 2022Article
- Identifying the Genetic Distance Threshold for Entiminae (Coleoptera: Curculionidae) Species Delimitation via COI Barcodes.Insects · 2022Article
- Longer mtDNA Fragments Provide a Better Insight into the Genetic Diversity of the Sycamore Lace Bug,Insects · 2022Article
- Excluding spatial sampling bias does not eliminate oversplitting in DNA-based species delimitation analyses.Ecology and evolution · 2021Article
- Structural Features and Phylogenetic Implications of Four New Mitogenomes of Caliscelidae (Hemiptera: Fulgoromorpha).International journal of molecular sciences · 2021Article
- The complete mitochondrial genome of the strawberry aphidMitochondrial DNA. Part B, Resources · 2021Article
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- A diagnostic LAMP assay for the destructive grapevine insect pest, phylloxera (Daktulosphaira vitifoliae).Scientific reports · 2020Article
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Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA barcode reference libraries linked to voucher specimens create new opportunities for high-throughput identification and taxonomic re-evaluations. This study provides a DNA barcode library for about 45% of the recognized species of Canadian Hemiptera, and the publically available R workflow used for its generation. The current library is based on the analysis of 20,851 specimens including 1849 species belonging to 628 genera and 64 families. These individuals were assigned to 1867 Barcode Index Numbers (BINs), sequence clusters that often coincide with species recognized through prior taxonomy. Museum collections were a key source for identified specimens, but we also employed high-throughput collection methods that generated large numbers of unidentified specimens. Many of these specimens represented novel BINs that were subsequently identified by taxonomists, adding barcode coverage for additional species. Our analyses based on both approaches includes 94 species not listed in the most recent Canadian checklist, representing a potential 3% increase in the fauna. We discuss the development of our workflow in the context of prior DNA barcode library construction projects, emphasizing the importance of delineating a set of reference specimens to aid investigations in cases of nomenclatural and DNA barcode discordance. The identification for each specimen in the reference set can be annotated on the Barcode of Life Data System (BOLD), allowing experts to highlight questionable identifications; annotations can be added by any registered user of BOLD, and instructions for this are provided.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.