ArticlePloS one2015
The Clostridium difficile Protease Cwp84 Modulates both Biofilm Formation and Cell-Surface Properties.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 86 citations in OpenAlex.
- From colonization to infection: Genomic evolution of Clostridioides difficile pathogenesis.Cell reports · 2026Review
- Cysteine proteases and how YabG fits into clan CD of the MEROPS database.Journal of bacteriology · 2025Review
- Extracellular DNA filaments associated with surface polysaccharide II give Clostridioides difficile biofilm matrix a network-like structure.NPJ biofilms and microbiomes · 2025Article
- Community-AcquiredInternational journal of molecular sciences · 2025Review
- Calcium modulates growth and biofilm formation of Lactobacillus acidophilus ATCC 4356 and Lactiplantibacillus plantarum ATCC 14917.Scientific reports · 2025Article
- The sigma factor σFrontiers in microbiology · 2025Article
- Article
- Genomic study of EuropeanMicrobial genomics · 2024Article
- Revealing roles of S-layer protein (SlpA) inMicrobiology spectrum · 2024Article
- Bacterial biofilm growth and perturbation by serine protease from Bacillus sp.Archives of microbiology · 2024Article
- A monoclonal antibody collection for C. difficile typing ?Gut pathogens · 2024Article
- Article
- Interactions of human milk oligosaccharides with the immune system.Frontiers in immunology · 2024Review
- Article
- Review of the Impact of Biofilm Formation on RecurrentMicroorganisms · 2023Review
- Biofilm Formation ofMicroorganisms · 2023Review
- Review
- Low-toxinEmerging microbes & infections · 2022Article
- Inhibition of In VitroMicroorganisms · 2022Article
- Impact of Subinhibitory Concentrations of Metronidazole on Morphology, Motility, Biofilm Formation and Colonization ofAntibiotics (Basel, Switzerland) · 2022Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
Abstract
Clostridium difficile is responsible for 15-20% of antibiotic-associated diarrheas, and nearly all cases of pseudomembranous colitis. Among the cell wall proteins involved in the colonization process, Cwp84 is a protease that cleaves the S-layer protein SlpA into two subunits. A cwp84 mutant was previously shown to be affected for in vitro growth but not in its virulence in a hamster model. In this study, the cwp84 mutant elaborated biofilms with increased biomass compared with the parental strain, allowing the mutant to grow more robustly in the biofilm state. Proteomic analyses of the 630Δerm bacteria growing within the biofilm revealed the distribution of abundant proteins either in cell surface, matrix or supernatant fractions. Of note, the toxin TcdA was found in the biofilm matrix. Although the overall proteome differences between the cwp84 mutant and the parental strains were modest, there was still a significant impact on bacterial surface properties such as altered hydrophobicity. In vitro and in vivo competition assays revealed that the mutant was significantly impaired for growth only in the planktonic state, but not in biofilms or in vivo. Taken together, our results suggest that the phenotypes in the cwp84 mutant come from either the accumulation of uncleaved SlpA, or the ability of Cwp84 to cleave as yet undetermined proteins.
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